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Series GSE19028 Query DataSets for GSE19028
Status Public on Mar 31, 2010
Title Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary To determine the effect of Sox17 overexpression in mouse embryonic stem (ES) cells, we performed gain-of-function analysis by generating ES cell lines carrying a doxycycline inducible FLAG-tagged Sox17 transgene. We treated Sox17-inducible ES cells with doxycycline, collected RNA and performed genome-wide transcriptional analysis. We found that genes invovled in adhesion function and basement membrane establishment were transcriptionally upregulated in ES cells upon induction of Sox17. We also observed downregulation in the transcription of genes involved in pathways known to be functionally important for ES cell pluripotency and self-renewal. However, Sox17 expression was not sufficient to rapidly down-regulate Sox2, Nanog, and Oct4.
 
Overall design Two independent doxycycline inducible Sox17-overexpressing mouse embryonic stem cells were derived. The genes expression changes in the Sox17-induced cells were compared to untreated (no doxycycline) controls and to control cells treated with or without doxycycline. The total RNA from these samples were amplified using Ambion Illumina TotalPrep RNA Amplification kit and arrayed on Illumina MouseRef8 v2 chips.
 
Contributor(s) Niakan KK, Sherwood RI
Citation(s) 20123909
Submission date Nov 13, 2009
Last update date Jun 14, 2018
Contact name Kathy Niakan
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Kevin Eggan
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM470907 Control KH2 cells treated with doxycycline
GSM470908 Control untreated KH2 cells
GSM470909 Sox17-inducible ES cells treated with doxycycline
Relations
BioProject PRJNA120625

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19028_RAW.tar 3.1 Mb (http)(custom) TAR
GSE19028_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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