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Series GSE190524 Query DataSets for GSE190524
Status Public on Jun 29, 2022
Title Transcriptomic analysis of sturgeon embryos in response to chemically induced DNA damage
Organism Acipenser ruthenus
Experiment type Expression profiling by high throughput sequencing
Summary In this study we show that the exposure to genotoxicants (10 nM camptothecin and 20 µM Olaparib) affects embryo development in a stage-dependent manner with early stages being the most sensitive to DNA damage. The transcriptomic and proteomic patterns changed significantly after exposure to genotoxicants compared to the control. Camptothecin and Olaparib downregulated metabolic pathways and developmental processes. Additionally, the exposure to genotoxicants led to upregulation of cell proliferation, neural precursor cell proliferation, generation of neurons, taxis and locomotion. Protein patterns were altered as well by DNA damage. The results of this study indicate that there is a strong correlation between phenotype formation and changes in transcriptomic and proteomic profiles.
 
Overall design We analyzed the effect of the genotoxins, CPT (camptothecin) and Ola (olaparib) on the transcriptome of the Acipenser ruthenus embryos during the sxposure period from 2 to 24 hpf (blastula); 24 to 48 hpf (gastrulation) and 48 to 72 hpf (neurulation).
 
Contributor(s) Gazo L, Sindelka R, Psenicka M, Abaffy P, Naraine R
Citation(s) 35742841
Submission date Dec 09, 2021
Last update date Jun 29, 2022
Contact name Radek Šindelka
Organization name Institute of Biotechnology, Czech Academy of Science, v.v.i.
Lab Laboratory of Gene Expression
Street address Průmyslová 595
City Vestec by Prague
ZIP/Postal code 25250
Country Czech Republic
 
Platforms (1)
GPL26119 Illumina NextSeq 500 (Acipenser ruthenus)
Samples (16)
GSM5725213 Ctrl 7 dpf rep 1
GSM5725214 Ctrl 7 dpf rep 2
GSM5725215 Ctrl 7 dpf rep 3
Relations
BioProject PRJNA787475

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Supplementary file Size Download File type/resource
GSE190524_Acipenser_ruthenus_non-normalized_counts.csv.gz 1.1 Mb (ftp)(http) CSV
GSE190524_Acipenser_ruthenus_normalized_counts.csv.gz 3.9 Mb (ftp)(http) CSV
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