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| Status |
Public on Dec 11, 2021 |
| Title |
Expression data from MDA-MB-231 breast cancer cell lines with DAXX Knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
DAXX, originally discovered as a context-dependent regulator of cell death or survival. Emerging evidence suggests that DAXX has an oncogenic role in diverse cancer types. However, the molecular mechanisms underlying DAXX's oncogenic function remain to be defined. We used Affymetrix Human Transcriptome Array 2.0 (HTA-2_0) to analyze genes that were up or downregulated upon DAXX knockdown.
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| Overall design |
The shRNA that target DAXX gene was transfected in human triple negative brest cancer MDA-MB-231 cell line by lentiviral particles and selected stable DAXX knockdown MDA-MB-231 cells. Scrambled control shRNA-transfected MDA-MB-231 cells were applying as control. Both stable DAXX knockdown and control MDA-MB-231 cells were seeded with 2 x 10^5 cells at 10 cm culture plate. Stable expression of shRNA was established through lentiviral transduction of MDA-MB-231 cell line and puromycin (2 µg/mL) selection. Three biological replicates were applied.
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| Contributor(s) |
Mahmud I, Liao D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 09, 2021 |
| Last update date |
Jan 23, 2023 |
| Contact name |
Daiqing Liao |
| E-mail(s) |
dliao@ufl.edu
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| Phone |
(352) 273-8188
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| Organization name |
University of Florida
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| Department |
Anatomy and Cell Biology
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| Lab |
480E
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| Street address |
2033 Mowry Rd
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA787619 |
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