|
| Status |
Public on Nov 19, 2009 |
| Title |
IL6 - IFNγ crosstalk experiment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment tested whether pretreatment of human microvascular endothelial cells (HMECs) with interferon-gamma (IFNγ) for 2h affected the transcriptional response to 1h exposure to interleukin-6 (IL-6).
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| Overall design |
Human microvascular endothelial cells in culture; exposure to IFNγ 1 ng/ml for 3 h alone, with IFNγ 1 ng/ml for 2 h and then with IL6 50 U/ml for 1 h, with IL6 50 U/ml alone, or with neither. 4 replicates for each condition. RNA isolation, Illumina expression arrays.
The supplementary file 'GSE19082_non-normalized_data.txt' contains non-normalized data for Samples GSM472434-GSM472449.
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| |
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| Contributor(s) |
Braam B, Rastmanesh M, Bluyssen H, Jie K, Wesseling S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 18, 2009 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Branko Braam |
| E-mail(s) |
bbraam@gmail.com
|
| Phone |
780 407 3322
|
| Fax |
780 407 7771
|
| URL |
http://www.nephrogenomics.net
|
| Organization name |
Univ. of Alberta
|
| Department |
Medicine / Nephrology
|
| Street address |
11-107 CSB
|
| City |
Edmonton |
| ZIP/Postal code |
T6C 3X6 |
| Country |
Canada |
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| Platforms (1) |
| GPL6104 |
Illumina humanRef-8 v2.0 expression beadchip |
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| Samples (16)
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| GSM472434 |
IFN-gamma 0ng/ml 2h + IL-6 0U/ml 1h CT ExpC05 |
| GSM472435 |
IFN-gamma 0ng/ml 2h + IL-6 50U/ml 1h CT ExpC05 |
| GSM472436 |
IFN-gamma 1ng/ml 2h + IL-6 0U/ml 1h CT ExpC05 |
| GSM472437 |
IFN-gamma 1ng/ml 2h + IL-6 50U/ml 1h CT ExpC05 |
| GSM472438 |
IFN-gamma 0ng/ml 2h + IL-6 0U/ml 1h CT Exp4 |
| GSM472439 |
IFN-gamma 0ng/ml 2h + IL-6 50U/ml 1h CT Exp4 |
| GSM472440 |
IFN-gamma 1ng/ml 2h + IL-6 0U/ml 1h CT Exp4 |
| GSM472441 |
IFN-gamma 1ng/ml 2h + IL-6 50U/ml 1h CT Exp4 |
| GSM472442 |
IFN-gamma 0ng/ml 2h + IL-6 0U/ml 1h CT Exp14 |
| GSM472443 |
IFN-gamma 0ng/ml 2h + IL-6 50U/ml 1h CT Exp14 |
| GSM472444 |
IFN-gamma 1ng/ml 2h + IL-6 0U/ml 1h CT Exp14 |
| GSM472445 |
IFN-gamma 1ng/ml 2h + IL-6 50U/ml 1h CT Exp14 |
| GSM472446 |
IFN-gamma 0ng/ml 2h + IL-6 0U/ml 1h CT Exp15 |
| GSM472447 |
IFN-gamma 0ng/ml 2h + IL-6 50U/ml 1h CT Exp15 |
| GSM472448 |
IFN-gamma 1ng/ml 2h + IL-6 0U/ml 1h CT Exp15 |
| GSM472449 |
IFN-gamma 1ng/ml 2h + IL-6 50U/ml 1h CT Exp15 |
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| Relations |
| BioProject |
PRJNA120493 |
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