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Series GSE191296 Query DataSets for GSE191296
Status Public on Jul 14, 2022
Title Emergence of sex-specific transcriptomes in a sexually-dimorphic brain nucleus
Organism Taeniopygia guttata
Experiment type Expression profiling by high throughput sequencing
Summary Sexual dimorphisms in the brain give rise to sex differences in physiology and behavior, yet we have limited understanding of the transcriptomic changes underlying their development. We evaluated developmental transcriptome dynamics for one of the most extreme neuroanatomical sexual dimorphisms in vertebrates - the songbird robust nucleus of the arcopallium (RA). RA is the telencephalic motor output nucleus of the song system. It grows monomorphically for the first few weeks posthatch, then continues growing in males but regresses in females, becoming 5-7 times larger in males. We quantified RA gene expression from monomorphic and dimorphic stages of development in both sexes. Mirroring the morphology, male and female transcriptomes initially resembled one other, then diverged markedly. Thousands of genes showed developmental regulation, corresponding to highly sex-specific biological processes. Males showed enrichments for neuronal growth and morphogenesis, synapse organization, metabolism, and voltage-gated ion channels. Females showed enrichments for cell polarity and differentiation, chemotaxis inhibition, gene silencing, and hormone and immune signaling. Notably, the majority of sex-biased genes in monomorphic RA were sex chromosome Z genes. These findings reveal a profound, sex-specific reorganization of RA’s transcriptome, providing novel insights into potential targets and drivers of sexually dimorphic neurodevelopment.
Overall design mRNA profiles of brain nucleus RA from 20 DPH and 50 DPH male and female zebra finches
Contributor(s) Friedrich SR, Nevue AA, Andrade AL, Velho TA, Mello CV
Citation(s) 35926465
Submission date Dec 20, 2021
Last update date Nov 04, 2022
Contact name Claudio V Mello
Organization name Oregon Health & Science University
Department Behavioral Neuroscience
Street address 3181 SW Sam Jackson Park Rd, L470
City Portland
State/province OR
ZIP/Postal code 97239
Country USA
Platforms (1)
GPL27119 Illumina NovaSeq 6000 (Taeniopygia guttata)
Samples (22)
GSM5743089 20 DPH female RA 1
GSM5743090 20 DPH male RA 1
GSM5743091 50 DPH male RA 1
BioProject PRJNA790926

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GSE191296_raw_gene_counts_matrix.tsv.gz 764.4 Kb (ftp)(http) TSV
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