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Series GSE19185 Query DataSets for GSE19185
Status Public on Nov 25, 2009
Title Low dose Leptin (25 ng/hr and 12.5 ng/hr) in ob/ob mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Ob/ob mice were given 0, 12.5 or 25 ng/hr leptin through an osmotic pump. After 12 days, livers RNA was prepared and illumina microarrays were done. We tested whether leptin can ameliorate diabetes independent of weight loss by defining the lowest dose at which leptin treatment of ob/ob mice reduces plasma [glucose] and [insulin]. We found that a leptin dose of 12.5 ng/hour significantly lowers blood glucose and that 25 ng/hour of leptin normalizes plasma glucose and insulin without significantly reducing body weight, thus establishing that leptin exerts its most potent effects on glucose metabolism. To find possible mediators of this effect, we profiled liver mRNA using microarrays and identified IGF Binding Protein 2 as being regulated by leptin with a similarly high potency. Over-expression of IGFBP2 by an adenovirus reversed diabetes in insulin resistant ob/ob, Ay/a and diet-induced obese mice (DIO), as well as insulin deficient streptozotocin-treated mice. Hyperinsulinemic clamp studies showed a three-fold improvement in hepatic insulin sensitivity following IGFBP2 treatment in ob/ob mice. These results show that IGFBP2 can regulate glucose metabolism, a finding with potential implications for the pathogenesis and treatment of diabetes.
Overall design n=3 for zero and 25 ng/hr. n=2 for 12.5 ng/hr
Contributor(s) Friedman JM, Hedbacker K
Citation(s) 20074524
Submission date Nov 25, 2009
Last update date Jun 14, 2018
Contact name Kristina Hedbacker
Organization name Rockefeller University
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM475609 0 ng/hr leptin_1
GSM475610 0 ng/hr leptin_2
GSM475611 0 ng/hr leptin_3
BioProject PRJNA120669

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Supplementary file Size Download File type/resource
GSE19185_RAW.tar 3.1 Mb (http)(custom) TAR
GSE19185_non-normalized_data.txt.gz 2.1 Mb (ftp)(http) TXT
GSE19185_normalized_averaged_data.txt.gz 3.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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