 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 31, 2010 |
| Title |
Serotonin stimulation of alveolar macrophages from wild type and 5-HT2C deficient animals |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene regulation upon serotonin stimulation was compared in wild-type and 5HT2C receptor kock-out mice.
Serotonin (5-HT), known as neuromodulator, regulates immune responses and inflammatory cascades. The expression and function of 5-HT receptors on alveolar macrophages (AM), which are the major fraction of pulmonary immune cells, remain elusive. Therefore we determined the expression of 5-HT type 2 receptors and investigated the effects evoked by stimulation with 5-HT in AM compared to alveolar epithelial cells (AEC). Quantitative PCR (qPCR) analysis revealed expression of the receptors 5-HT2A and 5-HT2B in AEC and of 5-HT2C in AM. In AM, 5-HT (10-5 M) induced a rise in intracellular calcium concentration ([Ca2+]i) that was initiated by release of Ca2+ from intracellular stores and depended on extracellular Ca2+ in a sustained phase. This 5-HT-induced increase in [Ca2+]i was not observed in AM treated with the 5-HT2C-selective inhibitor RS102221 and in AM derived from 5-HT2C-deficient mice. AM stimulated with 5-HT (10-5 M) showed increased expression of CCL2 (MCP-1) mRNA as assayed by qPCR at 4 h and augmented production of CCL2 protein as determined by dot-blot assay and ELISA at 24 h. Notably, in 5HT2C-deficient AM, CCL2 production was not affected by 5-HT treatment. Moreover, transcriptional responses to 5-HT exposure assayed by microarray experiments were only observed in AM from wild type animals but not in AM derived from 5-HT2C-deficient mice.
Taken together these data demonstrate the presence of functional 5-HT2C receptors on AM and suggest a role of 5-HT as novel modulator of AM function. These effects are exclusively driven by the 5-HT2C receptor, thereby providing the potential for selective intervention.
|
| |
|
| Overall design |
primary isolated murine alveolar macrophages were stimulated with serotonin
|
| |
|
| Contributor(s) |
Zaslona Z, Mikulski Z, Cakarova L, Hartmann P, Wilhelm J, Tecott L, Lohmeyer J, Kummer W |
| Citation(s) |
20495077 |
| Submission date |
Nov 27, 2009 |
| Last update date |
May 10, 2018 |
| Contact name |
Jochen Wilhelm |
| Organization name |
Uniklinikum Giessen
|
| Department |
ECCPS Microarray Unit
|
| Street address |
Gaffkystrasse 10
|
| City |
Giessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA120831 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19214_RAW.tar |
53.2 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...