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Series GSE19223 Query DataSets for GSE19223
Status Public on Nov 30, 2010
Title Strain-dependent Dissociation of Airway Hyper-responsiveness from Inflammation in a House Dust Mite Model of Asthma
Organism Mus musculus
Experiment type Expression profiling by array
Summary Gene expression analysis of Der p 1-treated mouse lungs.

We developed a new mouse model of allergic airway disease employing the house dust mite allergen Der p 1. This model produces qualitatively and quantitatively different effects in two commonly used inbred strains of mice, C57BL/6J and BALB/cJ, demonstrating that genetic background is an important determinant of response to Der p 1. To identify the genes and pathways associated with inflammation and pathophysiology, we used microarrays and found genes that were shared by both strains or were distinct. Among the latter, we identified three G-protein coupled receptors and a number of other genes and pathways whose expression patterns clearly distinguish the divergent physiologic responses of the two strains. We conclude that genetic background is a strong determinant of the type and intensity of response to Der p 1, including the degree of airway hyper-responsiveness and/or inflammation.
 
Overall design We analyzed whole lung RNAs from control (n=10-12) and Der p 1 treated (n=12-14) mice of two strains (BALB/cJ and C57BL/6J) using the Affymetrix Mouse Exon 1.0 ST platform. Array data was processed using Partek Genomics Suite software. Three techinical replicates were performed on a subset of samples.
 
Contributor(s) Kelada SN, Wilson MS, Kubalanza K, Tavarez U, Carpenter D, Borate B, Whitehead G, Maruoka S, Brass DM, Wynn TA, Evans CM, Schwartz DA, Collins FS
Citation(s) 21378263
Submission date Nov 30, 2009
Last update date Mar 06, 2018
Contact name Christopher Pan
E-mail(s) cpan@mail.nih.gov
Organization name NIH
Department NHGRI
Street address Rm 5228, Bldg 50, South Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (62)
GSM476159 mouse513-lungs-BALB/cJ Control-rep-1
GSM476160 mouse513-lungs-BALB/cJ Control-rep-2
GSM476161 mouse513-lungs-BALB/cJ Control-rep-3
Relations
BioProject PRJNA120845

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19223_RAW.tar 1.4 Gb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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