|
| Status |
Public on Dec 05, 2009 |
| Title |
Photoreceptor control of shoot meristem activity and leaf initiation |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
When Arabidopsis seedlings are grown in the dark, their shoot meristem remains in a repressed state and does not develop leaves. The tight control of leaf development by light provides the opportunity to analyse a fundamental plant developmental process in its very early stages. We have observed that activation of photoreceptors in dark-grown seedlings leads to a rapid and dramatic increase in mitotic activity in and around the shoot meristem. Cotyledons also exhibit an induction of cell cycle activity by light, but this consists of endoreduplication and occurs later. Many other processes take place upon induction by light, including the development of the photosynthetic apparatus and the synthesis of sunscreens, but these processes are expected to take place in both the leaf primordia and the cotyledons. We have used this information to carry out a whole transcriptome analysis of shoot meristem activation and early stages of leaf development by light. For this we dissected the shoot apical regions from seedlings grown in the dark or 1, 2, 6, 24, 48 or 72 h after transfer to light. We also dissected cotyledons from equivalent seedlings grown in the dark or 1 or 6 h after transfer to light. From these samples we obtained RNA used for hybridisations against the ATH1 GenChip.
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| |
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| Overall design |
16 samples were used in this experiment.
|
| |
|
| Contributor(s) |
Lopez-Juez E |
| Citation missing |
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| Submission date |
Dec 02, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (16)
|
| GSM477896 |
Lopez-Juez_1-1_Shoot-apex-dark_Rep1_ATH1 |
| GSM477897 |
Lopez-Juez_1-2_Shoot-apex-dark_Rep2_ATH1 |
| GSM477898 |
Lopez-Juez_1-3_Shoot-apex-1hr-light_Rep1_ATH1 |
| GSM477899 |
Lopez-Juez_1-4_Shoot-apex-1hr-light_Rep2_ATH1 |
| GSM477900 |
Lopez-Juez_1-5_Shoot-apex-2hr-light_Rep1_ATH1 |
| GSM477901 |
Lopez-Juez_1-6_Shoot-apex-6hr-light_Rep1_ATH1 |
| GSM477902 |
Lopez-Juez_1-7_Shoot-apex-6hr-light_Rep2_ATH1 |
| GSM477903 |
Lopez-Juez_1-8_Shoot-apex-24hr-light_Rep1_ATH1 |
| GSM477904 |
Lopez-Juez_1-9_Shoot-apex-48hr-light_Rep1_ATH1 |
| GSM477905 |
Lopez-Juez_1-10_Shoot-apex-72hr-light_Rep1_ATH1 |
| GSM477906 |
Lopez-Juez_1-11_Cotyledons-dark_Rep1_ATH1 |
| GSM477907 |
Lopez-Juez_1-12_Cotyledons-dark_Rep2_ATH1 |
| GSM477908 |
Lopez-Juez_1-13_Cotyledons-1hr-light_Rep1_ATH1 |
| GSM477909 |
Lopez-Juez_1-14_Cotyledons-1hr-light_Rep2_ATH1 |
| GSM477910 |
Lopez-Juez_1-15_Cotyledons-6hr-light_Rep1_ATH1 |
| GSM477911 |
Lopez-Juez_1-16_Cotyledons-6hr-light_Rep2_ATH1 |
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA120927 |
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