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Series GSE1927

Query DataSets for GSE1927

Status

Public on Nov 06, 2004

Title

Response of yeast to tomatidine treatment

Organism

Saccharomyces cerevisiae

Experiment type

Expression profiling by array

Summary

Genome-wide expression profiling was used to identify genes that showed altered expression in response to treatment with the fungistatic saponin tomatidine.

Overnight batch cultures of strain S288C were diluted to an optical density at 600nm of 0.1. After 1 hour of growth at 30°C, the compounds were added and the cultures were incubated for an additional 5 hours prior to harvesting. Control cultures were treated with the solvent dimethylformamide (DMF). Tomatidine treated cultures were grown in the presence of tomatidine (dissolved in DMF) at concentations of 7.5 uM and 15 uM.

Growth inhibition by the compounds was monitored by measuring the OD600nm. Cells were pelleted and frozen in liquid nitrogen. Acid-washed glass beads (0.5 mm diameter; Sigma) were added and the cells disrupted using two 20 s cycles at speed setting 6 in the Savant Bio 101 Fast Prep FP120. Total RNA was isolated using the Qiagen RNeasy kit (Qiagen, Inc., Valencia, CA, USA). Microarray hybridisation was performed using the Affymetrix GeneChip® Yeast genome S98 array using protocols described by Affymetrix, Inc. (Santa Clara, CA, USA) (as previously described (Zhu et al., 2001). Data were analyzed using Affymetrix® Microarray Suite version 5.0 software

Keywords: dose response

Contributor(s)

Simons V, Morrissey JP, Latijnhouwers M, Csukai M, Cleaver A, Osbourn A

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Submission date

Nov 04, 2004

Last update date

Jul 01, 2016

Contact name

Adam Alexander Cleaver

E-mail(s)

emad1@edac.fsnet.co.uk