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| Status |
Public on Dec 03, 2009 |
| Title |
Microarray gene expression profiling of aorta genes of APOE-deficient mice receiving the ACE inhibitor captopril |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray gene expression profiling of aorta genes of APOE-deficient mice receiving atherosclerosis treatment with the ACE inhibitor captopril.
Hypercholesterolemic APOE-deficient mice were used as a standard model of atherosclerosis to study gene expression changes during atherosclerosis treatment with the ACE inhibitor captopril. Microarray analysis was performed of whole aortas isolated from captopril-treated APOE-deficient mice relative to untreated APOE-deficient mice with overt atherosclerosis, and nontransgenic control mice. Microarray gene expression profiling revealed that captopril-mediated atherosclerosis prevention involved inhibition of aorta-infiltrating immune cells such as pro-atherogenic T lymphocytes and macrophages.
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| Overall design |
Microarray gene expression profiling was performed of whole aortas isolated from APOE-deficient mice with atherosclerosis relative to captopril-treated APOE-deficient mice, and nontransgenic control mice. Three study groups were analyzed, i.e. 8-months-old untreated APOE-deficient mice with overt atherosclerosis, age-matched APOE-deficient mice treated for 7 months with the angiotensin-converting enzyme (ACE) inhibitor, captopril (20 mg/kg in drinking water), and nontransgenic control C57BL/6J mice. Two biological replicates were made of each group, and total RNA of three aortas was pooled for one gene chip.
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| Contributor(s) |
Abd Alla J, Quitterer U |
| Citation(s) |
20504763 |
| Submission date |
Dec 02, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ursula Quitterer |
| E-mail(s) |
ursula.quitterer@pharma.ethz.ch
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| Phone |
+41-446329801
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| Organization name |
ETH Zurich
|
| Department |
Molecular Pharmacology
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
CH-8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM478606 |
Aorta_APOE-deficient with atherosclerosis_1 |
| GSM478607 |
Aorta_APOE-deficient with atherosclerosis_2 |
| GSM478608 |
Aorta_APOE-deficient and captopril treated_1 |
| GSM478609 |
Aorta_APOE-deficient and captopril treated_2 |
| GSM478610 |
Aorta_non-transgenic C57BL/6J control_1 |
| GSM478611 |
Aorta_non-transgenic C57BL/6J control_2 |
|
| Relations |
| BioProject |
PRJNA120369 |
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