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Series GSE19298 Query DataSets for GSE19298
Status Public on Feb 01, 2010
Title Gene expression timecourse in zebrafish whole eye in response to optic nerve crush
Organism Danio rerio
Experiment type Expression profiling by array
Summary It is well-established that neurons in the adult mammalian central nervous system are terminally differentiated and, if injured, will be unable to regenerate their connections. In contrast to mammals, zebrafish and other teleosts display a robust neuroregenerative response. Following optic nerve crush (ONX), retinal ganglion cells (RGC) regrow their axons to synapse with topographically correct targets in the optic tectum, such that vision is restored in ~21 days. What accounts for these differences between teleostean and mammalian responses to neural injury is not fully understood. A time course analysis of global gene expression patterns in the zebrafish eye after optic nerve crush can help to elucidate cellular and molecular mechanisms that contribute to a successful neuroregeneration.
We used microarrays to detail the global gene expression patterns underlying a successful regeneration or the optic nerve following injury.
Overall design Microarray analysis was performed on total RNA extracted from whole eye following optic nerve crush (ONX) or sham surgery at defined intervals (4, 12, & 21 days).
Contributor(s) McCurley AT, Callard GV
Citation(s) 20740047
Submission date Dec 03, 2009
Last update date Jan 25, 2018
Contact name Amy T McCurley
Organization name Boston University
Department Biology
Street address 5 Cummington Street
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (12)
GSM479019 SHAM eye at 4 days post-ONX, biological replicate 1
GSM479020 SHAM eye at 4 days post-ONX, biological replicate 2
GSM479021 ONX eye at 4 days post-ONX, biological replicate 1
BioProject PRJNA120883

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Supplementary file Size Download File type/resource
GSE19298_RAW.tar 25.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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