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| Status |
Public on May 06, 2022 |
| Title |
A single-cell transcriptomic atlas characterizes the silk-producing organ in the silkworm |
| Organism |
Bombyx mori |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The silk gland (SG) of the domesticated silkworm Bombyx mori, an economically important insect that has been used for silk production for over 5000 years, is a remarkable organ that produces vast amounts of silk with exceptional properties . Little is known about which SG cells execute silk protein synthesis and its precise spatiotemporal control. Here, we used single-cell RNA-seq to build a comprehensive cell atlas of the B. mori SG, consisting of 14,972 high-quality cells representing 10 distinct cell types, in three early developmental stages. We annotated all 10 cell types and determined their distributions in each region of the SG, decoded their developmental trajectory and gene-switch status, and discovered marker genes involved in the regulation of SG development and silk protein synthesis. Our study reveals the high heterogeneity of B. mori SG cells and their gene expression dynamics for the first time, affording a deeper understanding of silk-producing organs at the single-cell level .
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| Overall design |
SG cells collected from B. mori at three developmental stages (8 days post-egg laying, E8D; 1L1D; and first larval molting, 1LM), using single-cell RNAseq
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| Contributor(s) |
Xu H |
| Citation(s) |
35680954 |
| Submission date |
Jan 08, 2022 |
| Last update date |
Jun 24, 2022 |
| Contact name |
Hanfu Xu |
| E-mail(s) |
xuhf@swu.edu.cn
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| Organization name |
State Key Laboratory of Silkworm Genome Biology Southwest University
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| Street address |
2 Tiansheng Road, Beibei District
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| City |
Chongqing |
| ZIP/Postal code |
400715 |
| Country |
China |
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| Platforms (1) |
| GPL29210 |
Illumina NovaSeq 6000 (Bombyx mori) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA795657 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE193279_RAW.tar |
47.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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