NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE19421 Query DataSets for GSE19421
Status Public on Dec 11, 2009
Title Profile of microRNA during adipogenesis regulating by microRNA and WNT signaling
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary To investigate miRNAs that implicate the process of adipogenesis by interacting with canonical Wnt/β-catenin signaling pathway, we constructed two cell models at first, and then investigated the expression profile of microRNAs by using Microarray. Based on the data of high throughput microarray, we identified 18 miRNAs which might promote adipogenesis by repressing WNT signaling, including mir-210, mir-148a, mir-194, mir-322 etc. On the other hand, we also identified 29 miRNAs which might repress adipogenesis by activation of WNT signaling, including mir-344, mir-27, and mir-181. The target genes involved in WNT signaling pathway of these identified miRNAs were also predicted through online tools.
 
Overall design Two cell models of 3T3-L1, i.e. activation and suppression of WNT signaling including four samples, i.e. preadipocytes, mature adipocytes (MDI induction), Lithium treated preadipocytes, MDI induction of Lithium treated preadipocytes. Each sample was replicated in triplicate.
 
Contributor(s) Niu YN, Mo DL, Chen WQ, Wang QW, Xiao SQ, Xie Y, Li AN, Li J, Zhao X, He ZY
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 10, 2009
Last update date Mar 21, 2012
Contact name Qin Limei
E-mail(s) wyqbj2004@163.com
Organization name SunYatsen University
Street address North Third road
City Guangzhou
ZIP/Postal code 510006
Country China
 
Platforms (1)
GPL9756 Agilent-021828 Unrestricted Mouse miRNA Microarray (miRNA_105_Dec08)
Samples (12)
GSM482975 preadipocytes A-1
GSM482976 preadipocytes A-2
GSM482977 preadipocytes A-3
Relations
BioProject PRJNA120359

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19421_RAW.tar 9.3 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap