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| Status |
Public on Apr 11, 2010 |
| Title |
Expression data from Fog1+/+ and Fog1 ki/ki mouse megakaryocyte-erythroid progenitors (MEP). |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcription co-factor FOG1 interacts with the chromatin remodeling complex NuRD to mediate gene activation and gene repression during hematopoiesis. We have generated mice with a targeted mutation in the endogenous Fog1 locus that results in an N-ternimal mutation in FOG1 that disrupts the interaction with NuRD.
We used gene expression microarrays to explore the global transcriptional programs regulated by FOG1 and NuRD in megakaryocyte-erythroid progenitors (MEP) to aid in understanding its role during hematopoiesis.
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| Overall design |
Flow cytometry was used to isolate Megakaryocyte-Erythroid progenitors (MEP) from murine bone marrow. MEP were identified as Lineage neg, kit pos, Sca1 neg, CD34 neg, FcgrII/III low cells.
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| |
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| Contributor(s) |
Gregory GD, Zhang Z, Blobel GA |
| Citation(s) |
20065294 |
| Submission date |
Dec 15, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Gregory D Gregory |
| E-mail(s) |
gregdgregory@gmail.com
|
| Phone |
215-590-4461
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Pediatrics/Hematology
|
| Lab |
Gerd Blobel
|
| Street address |
3615 Civic Center Blvd, ARC 315
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA122415 |
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