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| Status |
Public on Dec 19, 2009 |
| Title |
Transcriptional program of terminal granulocytic differentation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To characterize the transcriptional program that governs terminal granulocytic differentation in vivo, we performed comprehensive microarray analysis of human bone marrow population highly enriched for promyelocytes, myelocytes / metamyelocytes and neotrophils.
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| Overall design |
Bone marrow and peripheral blood samples were collected from healthy individuals in parallel. Cell populations representing successive stages of terminal granulocytic differentation were isolated from human bone marrow samples by 2-layer density gradient centrifugation, neutrophils were collected from peripheral blood using 1-layer density gradient centrifugation. All populations were depleted of nongranulocytic cells by immunomagnetic sorting.
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| |
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| Contributor(s) |
Theilgaard-Monch K, Marstrand TT |
| Citation(s) |
25645918 |
| Submission date |
Dec 18, 2009 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Troels Torben Marstrand |
| E-mail(s) |
tmarstra@princeton.edu
|
| Organization name |
Princeton University
|
| Street address |
Lewis-Sigler Institute Princeton University
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08540 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA122397 |
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