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Series GSE19576 Query DataSets for GSE19576
Status Public on Dec 24, 2009
Title Prkar1a haploinsufficiency leads to an overall increase in tumors caused by other genetic defects or chemical induction
Organism Mus musculus
Experiment type Expression profiling by array
Summary [original title] Prkar1a haploinsufficiency in mice leads to an overall increase in tumors caused by other genetic defects or chemical induction

We investigated the Prkar1a+/- mice when bred within the Rb1+/- or Trp53+/- genetic backgrounds, or treated with a 2-step skin carcinogenesis protocol. Prkar1a+/-Trp53+/- mice developed more bone sarcomas than Trp53+/- mice (p<0.05) and Prkar1a+/-Rb1+/- mice grew more and larger pituitary and thyroid tumors than Rb1+/- mice. All mice with double heterozygosity had significantly reduced life-spans compared with their single-heterozygous counterparts. Finally, Prkar1a+/- mice developed more papillomas than wild-type animals when treated with a 2-step skin carcinogenesis protocol. A whole-genome transcriptome profiling of tumors produced by all three models identified Wnt signaling as the main pathway activated by abnormal cAMP signaling in these tissues, along with (expected) cell cycle gene abnormalities, all confirmed by qRT-PCR array and immunohistochemical analyses.
 
Overall design Total RNA obtained from skin papillomas from Prkar1a+/- mice were compared to those obtained from WT mice.
Total RNA obtained from pituitary and thyroid tumors from Rb1+/- mice were compared to those samples obtained from Prkar1a+/- Rb1+/- mice. Total RNA obtained from sarcomas from Tp53+/- mice were compared to those samples obtained from Prkar1a+/- Tp53+/- mice.
 
Contributor(s) Almeida MQ, Nesterova M, Stratakis CA, Cheadle C, Watkins T
Citation(s) 20080939
Submission date Dec 18, 2009
Last update date Jun 14, 2018
Contact name Madson Q Almeida
E-mail(s) madsonalmeida@usp.br
Phone 301 451 3768
Fax 301 402 0574
Organization name NIH
Department Section on Genetics and Endocrinology / NICHD
Lab Dr. Stratakis Lab
Street address 10 Center Drive, MSC 1103
City Bethesda
State/province MD
ZIP/Postal code 20892-1103
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (23)
GSM490179 WT skin papilloma -1
GSM490180 WT skin papilloma -2
GSM490181 Prkar1a+/- skin papilloma -1
Relations
BioProject PRJNA122465

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19576_RAW.tar 3.1 Mb (http)(custom) TAR
GSE19576_non-normalized_pituitarythyroidtumors_sarcomas.txt.gz 1.4 Mb (ftp)(http) TXT
GSE19576_non-normalized_skinpapillomas.txt.gz 440.8 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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