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| Status |
Public on Aug 01, 2010 |
| Title |
Mouse embryonic kidneys (E13.5): HDAC inhibitor (HDACi) treated vs. Control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of cultured CD1 mouse embryonic kidneys (E13.5) comparing HDACi-treated kidneys with control drug-treated kidneys. Studies in our lab showed that pharmacological inhibition of HDAC activity in ex-vivo cultured metanephroi results in extensive defects in kidney development, including impaired UB branching, tubulogenesis, and glomerulogenesis, accompanied by cell cycle arrest and apoptosis.The goal of the microarray analysis was to elucidate the morphogenetic pathways affected by HDACi.
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| Overall design |
Two-condition experiment, HDACi-treated E13.5 kidneys (Scriptaid 2.0 μg/ml x 6hrs) vs. Control drug-treated E13.5 kidneys (Nullscript 2.0 μg/ml x 6hrs). Biological replicates: 3 control replicates, 3 HDACi-treated replicates. Two-color Agilent 4x44k chips with dye-swap on 2 of 4 arrays.
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| Contributor(s) |
Chen S, Bachvarov D, El-Dahr SS |
| Citation(s) |
21778236 |
| Submission date |
Dec 18, 2009 |
| Last update date |
May 10, 2018 |
| Contact name |
Samir S El-Dahr |
| E-mail(s) |
seldahr@tulane.edu
|
| Organization name |
Tulane University School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Dr. El-Dahr
|
| Street address |
1430 Tulane Ave
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
| Samples (6)
|
| GSM488043 |
HDACi-treated kidneys vs. control drug-treated kidneys Replicate 1 |
| GSM488044 |
control drug-treated kidneys vs. HDACi-treated kidneys Replicate 1 |
| GSM488045 |
HDACi-treated kidneys vs. control drug-treated kidneys Replicate 2 |
| GSM488046 |
control drug-treated kidneys vs. HDACi-treated kidneys Replicate 2 |
| GSM488047 |
HDACi-treated kidneys vs. control drug-treated kidneys Replicate 3 |
| GSM488048 |
control drug-treated kidneys vs. HDACi-treated kidneys Replicate 3 |
|
| Relations |
| BioProject |
PRJNA122353 |
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