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Series GSE19588 Query DataSets for GSE19588
Status Public on Apr 08, 2011
Title Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network (2)
Organism Mus musculus
Experiment type Expression profiling by array
Summary The embryonic stem (ES) cell transcriptional and epigenetic networks are critical for the maintenance of ES cell self-renewal. It remains unclear whether interactions. Here we show that a core member of the Set/MLL complex, WDrepeat protein-5 (Wdr5), is a novel regulator of self-renewal and partners with Oct4 to maintain an intact transcriptional network and for efficient formation of induced pluripotent stem (iPS) cells. Moreover, we provide evidence that Oct4 interacts with chromatin components and is indispensible for epigenetic regulation. These findings suggest an integrated transcriptional and epigenetic model, mediated through Wdr5, for the maintenance of ES cell self-renewal and somatic cell reprogramming.

Differential gene expressions upon down-regulation of Wdr5 was analysed by comparing Wdr5R4 –dox (triplicate) and LucR +dox (duplicate) RNA samples

Gene expression changes upon down-regulation of Oct4 was analysed by comparing Oct4 shRNA (duplicate) and GFP shRNA (duplicate) after 4day transfection
Overall design Wdr5: 5 samples are analyzed: Luciferase (Luc, 2 replicates) and Wdr5 with no dox (minusDox, 3 replicates)
Oct4-GFP: 4 samples are analyzed: Oct4 shRNA (Oct4, 2 replicates) and GFP shRNA (GFP, 2 replicates)
Contributor(s) Ang Y, Tsai S, Lee D, Monk J, Su J, Ratnakumar K, Darr H, Chang B, Rendl M, Schaniel C, Bernstein E, Lemischka IR
Citation(s) 21477851
Submission date Dec 20, 2009
Last update date Jun 14, 2018
Contact name Jonathan Monk
Phone 9716457785
Organization name Mt. Sinai School of Medicine
Department Gene and Cell Medicine
Lab Lemischka
Street address One Gustave L. Levy Place
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (2)
GPL6481 Illumina mouse-6 v1.1 expression beadchip [Array_Address_Id version]
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (9)
GSM488133 Luciferase replicate 1
GSM488134 Luciferase replicate 2
GSM488135 Rescue Clone Minus Dox replicate 1
BioProject PRJNA122365

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19588_OCT4_genes_normalized.txt.gz 2.5 Mb (ftp)(http) TXT
GSE19588_Oct4-GFP-FC.txt.gz 1.1 Mb (ftp)(http) TXT
GSE19588_Oct4-GFP-non-normalized.txt.gz 1005.8 Kb (ftp)(http) TXT
GSE19588_RAW.tar 3.1 Mb (http)(custom) TAR
GSE19588_WDR5_genes_normalized.txt.gz 1.7 Mb (ftp)(http) TXT
GSE19588_Wdr5_FC.txt.gz 621.7 Kb (ftp)(http) TXT
GSE19588_Wdr5_non-normalized.txt.gz 564.4 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record
Processed data are available on Series record
Processed data provided as supplementary file
Raw data provided as supplementary file

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