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Series GSE19624 Query DataSets for GSE19624
Status Public on Sep 01, 2010
Title Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium
Organism Mus musculus
Experiment type Expression profiling by array
Summary Endothelin-1 (ET-1), an endothelium-derived vasoconstrictor peptide, plays a role in the pathophysiology of cardiovascular disease. Transgenic mice that overexpress human preproET-1 selectively in the endothelium (eET-1) exhibit endothelial dysfunction, hypertrophic remodeling and vascular inflammation of resistance-size arteries in the absence of blood pressure elevation. To understand the mechanisms whereby ET-1 induces vascular damage, vascular gene expression using DNA microarrays was employed. RNA from mesenteric arteries of female and male young (6-7 weeks) and mature (6-8 months) eET-1 and wild type (WT) mice was isolated and changes in gene expression were determined by genome-wide expression profiling using Illumina microarray. This study revealed a set of genes potentially regulated by ET-1, which might be implicated in ET-1 induced-vascular damage.
 
Overall design Samples 1-8: Total RNA obtained from the entire mesenteric arterial tree of young (6-7 weeks) female preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.

Samples 9-16: Total RNA obtained from the entire mesenteric arterial tree of mature (6-8 months) female preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.

Samples 17-24: Total RNA obtained from the entire mesenteric arterial tree of young (6-7 weeks) male preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.

Samples 25-32: Total RNA obtained from the entire mesenteric arterial tree of mature (6-8 months) male preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.
 
Contributor(s) Simeone SM, Paradis P, Schiffrin EL
Citation(s) 21045115
Submission date Dec 23, 2009
Last update date Jun 14, 2018
Contact name Stefania Simeone
E-mail(s) stefania.simeone@mail.mcgill.ca
Phone 514-340-8222
Organization name Lady Davis Institute for Medical Research
Department Hypertension and Vascular Research Unit
Lab Dr. Ernesto L Schiffrin
Street address 3755 Côte Ste-Catherine Road
City Montreal
State/province Quebec
ZIP/Postal code H3T 1E2
Country Canada
 
Platforms (2)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
GPL8389 Illumina Mouse-8 Expression BeadChip
Samples (32)
GSM489305 mesenteric arterial tree, eET-1 mouse, replicate 1, female young
GSM489306 mesenteric arterial tree, eET-1 mouse, replicate 2, female young
GSM489307 mesenteric arterial tree, eET-1 mouse, replicate 3, female young
Relations
BioProject PRJNA122579

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19624_RAW.tar 3.1 Mb (http)(custom) TAR
GSE19624_SampleProbeProfile_Female_Mature.txt.gz 5.7 Mb (ftp)(http) TXT
GSE19624_SampleProbeProfile_Female_Young.txt.gz 2.5 Mb (ftp)(http) TXT
GSE19624_SampleProbeProfile_Male_Mature.txt.gz 6.5 Mb (ftp)(http) TXT
GSE19624_SampleProbeProfile_Male_Young.txt.gz 5.7 Mb (ftp)(http) TXT
GSE19624_female_mature_RAW.txt.gz 1.3 Mb (ftp)(http) TXT
GSE19624_female_young_RAW.txt.gz 1.3 Mb (ftp)(http) TXT
GSE19624_male_mature_RAW.txt.gz 1.3 Mb (ftp)(http) TXT
GSE19624_male_young_RAW.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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