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| Status |
Public on Nov 16, 2004 |
| Title |
Portrait of transcriptional responses to ultraviolet and ionizing radiation in human cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Peripheral blood lymphocytes from a total of 15 healthy individuals without history of cancer (NoCa) were immortalized with Epstein-Barr virus.
Cells were exposed to mock treatment (Mock), ultra-violet radiation (UV), or ionizing radiation (IR). For UV radiation treatment, cells were exposed to 10 J/m^2 and harvested for RNA 24 hours later. For IR treatment, cells were exposed to 5 Gy of IR and harvested for RNA 4 hours later. For example, NoCa1-Mock refers to cells from healthy patient 1 exposed to mock treatment. The published manuscript (NAR 32:4786, 2004) can be found at http://nar.oupjournals.org/cgi/content/abstract/32/16/4786
Data were analyzed with Affymetrix MAS version 4.0.
Normalization – A reference data set was generated by averaging the expression of each gene over all data sets. The data for each hybridization were compared with the reference data set in a cube root scatter plot. A linear least-squares fit to the cube root scatter plot was then used to normalize each hybridization.
Keywords: repeat sample
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| Contributor(s) |
Rieger KE, Chu G, Hong W, Tusher VG, Tang J, Tibshirani R |
| Citation(s) |
15356296 |
| Submission date |
Nov 16, 2004 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Wan-Jen Hong |
| E-mail(s) |
wanjen@stanford.edu
|
| Phone |
650-725-6441
|
| Organization name |
Stanford University School of Medicine
|
| Department |
Medicine/Biochemistry
|
| Lab |
Gilbert Chu
|
| Street address |
269 Campus Dr., CCSR Room 1140
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA90923 |
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