|
| Status |
Public on Mar 04, 2010 |
| Title |
Neuronal MeCP2 is expressed at near histone-octamer levels and globally alters the chromatin state |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
We report the application of single molecule-based sequencing technology for high-throughput genom-wide mapping of MeCP2 binding and DNA methylation in mouse brain and cerebellum respectively. We find a good correlation between MeCP2 occupancy and methyl-CpG density and depletion of MeCP2 binding at CpG Islands, the majority of which are constitutively hypomethylated. This study provides a comprehensive characterisation of the genome wide distribution of MeCP2.
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| |
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| Overall design |
Examination of MeCP2 occupancy in mouse brain and the distribution of methylation in mouse cerebellum.
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| |
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| Contributor(s) |
Skene PJ, Illingworth RS, Webb S, Kerr A, James KD, Turner DJ, Andrews R, Bird AP |
| Citation(s) |
20188665 |
| Submission date |
Jan 07, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Adrian P Bird |
| E-mail(s) |
A.Bird@ed.ac.uk
|
| Phone |
0131 6508695
|
| Fax |
0131 6505379
|
| Organization name |
The University of Edinburgh
|
| Department |
WTCCB
|
| Lab |
Bird Lab
|
| Street address |
Mayfield Road
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP002061 |
| BioProject |
PRJNA122117 |
