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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 21, 2010 |
| Title |
Prolonged IL-2R alpha expression on virus-specific CD8+ T cells favors terminal effector differentiation in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CD25, the high affinity interleukin-2 (IL-2) receptor alpha-chain, is rapidly upregulated by antigen-specific CD8+ T cells after T cell receptor stimulation. We demonstrated that during an acute viral infection, CD25 expression was dynamic, and a subset of virus-specific CD8+ T cells sustained CD25 expression longer than the rest. Examination of the in vivo fate of effector CD8+ T cells exhibiting differential responsiveness to IL-2 revealed that CD25lo cells, which were relatively less sensitive to IL-2, preferentially upregulated CD127 and CD62L and gave rise to the functional long-lived memory pool. In contrast, CD25hi cells that accumulate enhanced IL-2 signals, proliferated more rapidly, were prone to apoptosis, exhibited a more pronounced effector phenotype, and appeared to be terminally differentiated. Sustained IL-2 receptor signaling resulted in increased CD8+ T cell proliferation, higher granzyme B expression and exaggerated contraction after antigen clearance. These data support the hypothesis that prolonged IL-2 signals during priming promote terminal effector differentiation of CD8+ T cells.
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| Overall design |
An important question in memory development is understanding the differences between effector CD8 T cells that die versus effector cells that survive and give rise to memory cells. In this study we have performed genomic profiling of terminal effectors and memory precursors as defined by CD25 heterogeneity, towards better understanding the generation of these subsets. The two effector subsets were FACS purified based on the amount of cell surface CD25 expression into CD25lo and CD25hi subsets during the early expansion phase (Days 3-4 post-infection) and analyzed for their gene expression profiles (by genome-wide microarray analyses).
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| Contributor(s) |
Kalia V, Sarkar S |
| Citation(s) |
20096608 |
| Submission date |
Jan 11, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Vandana Kalia |
| E-mail(s) |
vkalia@emory.edu
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| Phone |
404-660-9784
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| Fax |
404-727-4461
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| Organization name |
Emory University
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| Department |
Microbiology and Immunology
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| Street address |
1510 Clifton Road
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (11)
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| GSM495040 |
Naïve P14 CD8 T cells, Biological Replicate 1 |
| GSM495041 |
Naïve P14 CD8 T cells, Biological Replicate 2 |
| GSM495042 |
Naïve P14 CD8 T cells, Biological Replicate 3 |
| GSM495043 |
CD25 Lo CD8 T cells, Biological Replicate 1 |
| GSM495044 |
CD25 Lo CD8 T cells, Biological Replicate 2 |
| GSM495045 |
CD25 Lo CD8 T cells, Biological Replicate 3 |
| GSM495046 |
CD25 Lo CD8 T cells, Biological Replicate 4 |
| GSM495047 |
CD25 Hi CD8 T cells, Biological Replicate 1 |
| GSM495048 |
CD25 Hi CD8 T cells, Biological Replicate 2 |
| GSM495049 |
CD25 Hi CD8 T cells, Biological Replicate 3 |
| GSM495050 |
CD25 Hi CD8 T cells, Biological Replicate 4 |
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| Relations |
| BioProject |
PRJNA122155 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19825_RAW.tar |
39.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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