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| Status |
Public on Jan 13, 2010 |
| Title |
Whole brains and frontal lobes of rat offsprings exposed perinatally to low doses of 2,2',4,4'-tetrabromodiphenyl ether |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of developmental toxicity of environmental toxin 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) at the level of gene expression. Dams of Wistar rats were exposed to environmentaly relevant doses of BDE-47 at gestation and lactation. Gene expression in total brains and frontal lobes was analysed in offspring on postnatal day 10.
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| Overall design |
Dams were randomly distributed among three groups (3 dams per group) and exposed by caudal injection (0.3 ml/kg body weight) on GD 15, PND 1 and PND 5. Dams of the control group received vehicle: ethanol 95%, Cremophor EL, and sterile water (1:1:8, v/v); dams of exposed group 1 received 0.002 mg/kg body weight (BW) of BDE-47 in vehicle, and dams of exposed group 2 received 0.2 mg/kg body weight (BW) of BDE-47 in vehicle. One female pup per litter was selected randomly and sacrificed by decapitation on PND 10. Total RNA was extracted from total brain and frontal lobe samples of these pups and used for microarray analysis.
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| Contributor(s) |
Suvorov A, Takser L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 12, 2010 |
| Last update date |
Jul 29, 2014 |
| Contact name |
Alexander Suvorov |
| E-mail(s) |
alexander.suvorov@usherbrooke.ca
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| Organization name |
Université de Sherbrooke
|
| Street address |
3001, 12e avenue Nord
|
| City |
Sherbrooke |
| State/province |
Quebec |
| ZIP/Postal code |
J1H 5N4 |
| Country |
Canada |
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| Platforms (1) |
| GPL6101 |
Illumina ratRef-12 v1.0 expression beadchip |
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| Samples (18)
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| GSM496385 |
Brain of pup from control group rep1 |
| GSM496386 |
Brain of pup from control group rep2 |
| GSM496387 |
Brain of pup from control group rep3 |
| GSM496388 |
Brain of pup exposed to 0.002 mg/kg BW BDE-47 rep1 |
| GSM496389 |
Brain of pup exposed to 0.002 mg/kg BW BDE-47 rep2 |
| GSM496390 |
Brain of pup exposed to 0.002 mg/kg BW BDE-47 rep3 |
| GSM496391 |
Brain of pup exposed to 0.2 mg/kg BW BDE-47 rep1 |
| GSM496392 |
Brain of pup exposed to 0.2 mg/kg BW BDE-47 rep2 |
| GSM496393 |
Brain of pup exposed to 0.2 mg/kg BW BDE-47 rep3 |
| GSM496394 |
Brain frontal lobes of pup from control group rep1 |
| GSM496395 |
Brain frontal lobes of pup from control group rep2 |
| GSM496396 |
Brain frontal lobes of pup from control group rep3 |
| GSM496397 |
Brain frontal lobes of pup exposed to 0.002 mg/kg BW BDE-47 rep1 |
| GSM496398 |
Brain frontal lobes of pup exposed to 0.002 mg/kg BW BDE-47 rep2 |
| GSM496399 |
Brain frontal lobes of pup exposed to 0.002 mg/kg BW BDE-47 rep3 |
| GSM496400 |
Brain frontal lobes of pup exposed to 0.2 mg/kg BW BDE-47 rep1 |
| GSM496401 |
Brain frontal lobes of pup exposed to 0.2 mg/kg BW BDE-47 rep2 |
| GSM496402 |
Brain frontal lobes of pup exposed to 0.2 mg/kg BW BDE-47 rep3 |
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| Relations |
| BioProject |
PRJNA121955 |
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