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| Status |
Public on Mar 31, 2010 |
| Title |
Expression data from HMC-1 mast cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We demonstrate that the G protein Gi3 is the cellular target of the adenosine A3 receptor (A3R). By using a cell permeable peptide comprising the C-terminal end of Gαi3 fused to an importation sequence (ALL1) as a selective inhibitor of Gi3 signaling, we show that by coupling to Gi3, the A3R stimulates multiple signaling pathways in human mast cells, leading to upregulation of cytokines, chemokines and growth factors.Following contact with activated T cell membranes, endogenous adenosine binds to and activates the A3R, resulting in Gi3-mediated signaling. Specifically, the majority of ERK1/2 signaling initiated by contact with activated T cell membranes, is mediated by Gi3, giving rise to ALL1-inhibitable cellular responses. These results unveil the physiological GPCR that couples to Gi3 and establish the important role played by this G-protein in inflammatory conditions that involve adenosine-activated mast cells.
We used microarrays to detail the effect of ALL1 on gene expression of HMC-1 cells activated directly by the A3 receptor, or by contact with activated T cell membranes.
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| Overall design |
The experiment consisted of 6 treatments in duplicates, a total of 12 samples. The cells were activated via two different activation pathways (A3 and T membranes), with or without pretreatment with the drug ALL1.
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| Contributor(s) |
Baram D, Sagi-Eisenberg R, Dekel O |
| Citation(s) |
20190146 |
| Submission date |
Jan 14, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Dana Baram |
| E-mail(s) |
danabaram1@gmail.com
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| Organization name |
Tel-Aviv University
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| Street address |
P.O. Box 39040
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| City |
Tel-Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (12)
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| GSM497180 |
Incubated with ALL1 rep 2 |
| GSM497181 |
Activated by the A3 receptor rep 1 |
| GSM497182 |
Activated by the A3 receptor rep 2 |
| GSM497183 |
Preincubated with ALL1 and activated by the A3 receptor rep 1 |
| GSM497184 |
Preincubated with ALL1 and activated by the A3 receptor rep 2 |
| GSM497185 |
Activated by activated T cell membranes rep 1 |
| GSM497186 |
Activated by activated T cell membranes rep 2 |
| GSM497187 |
Preincubated with ALL1 and activated by activated T cell membranes rep 1 |
| GSM497188 |
Preincubated with ALL1 and activated by activated T cell membranes rep 2 |
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| Relations |
| BioProject |
PRJNA122029 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19888_RAW.tar |
21.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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