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Series GSE19889 Query DataSets for GSE19889
Status Public on May 16, 2010
Title Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa (Illumina)
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary In higher eukaryotes, histone methylation is involved in the maintenance of cellular identity during somatic development. During spermatogenesis, Since most nucleosomes are replaced by protamines during spermatogenesis . Iit is therefore unclear whether if histone modifications function in paternal transmission of epigenetic information. Here we show that H3K4 di-methylation (H3K4me2) and H3K27 tri-methylation (H3K27me3), two modifications important for Trithorax and Polycomb-mediated gene regulation, display methylation-specific distributions at regulatory regions in human spermatozoa. H3K4 dimethylation H3K4me2-marksed promoters of genes relevant control gene functions in spermatogenesis and cellular homeostasis suggesting that this mark reflects germline transcription. In contrast, H3K27 trimethylation (H3K27me3) marks promoters of key developmental regulators in sperm like in somatic cells. Promoters of orthologous genes are similarly modified in mouse spermatozoa. Further, particularly genes with extensive H3K27me3 coverage around transcriptional start sites are never expressed during male and female gametogenesis, nor in pre-implantation embryos. These data are compatible with a function for Polycomb in repressing somatic determinants across generations. Importantly, however, we observe only modest selective retention of nucleosomes at regulatory regions in human sperm suggesting that paternal transmission of H3K27me3-encoded epigenetic information may be subjected to variegation.
 
Overall design Identification of nucleosome containing regions in 6 human sperm samples
 
Contributor(s) Hisano M, Erkek S, Stadler MB, Peters AH
Citation(s) 20473313
Submission date Jan 14, 2010
Last update date May 15, 2019
Contact name Antoine Peters
E-mail(s) antoine.peters@fmi.ch
Organization name Friedrich Miescher Institute for Biomedical Research (FMI)
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (7)
GSM497189 Donor 1 biological replicate a
GSM497190 Donor 1 biological replicate b
GSM497191 Donor 2 biological replicate a
This SubSeries is part of SuperSeries:
GSE19892 Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa
Relations
SRA SRP002182
BioProject PRJNA124021

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Supplementary file Size Download File type/resource
GSE19889_RAW.tar 51.1 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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