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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 05, 2010 |
| Title |
MicroRNA function is globally suppressed in mouse oocytes and early embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dicer, which is required for the processing of both microRNAs (miRNAs) and small interfering RNAs (siRNAs), is essential for oocyte maturation. Oocytes express both miRNAs and endogenous siRNAs (endo-siRNAs). To determine whether the abnormalities in Dicer knockout oocytes during meiotic maturation are secondary to the loss of endo-siRNAs and/or miRNAs, we deleted Dgcr8, which encodes a RNA binding protein specifically required for miRNA processing. In striking contrast to Dicer, Dgcr8 deficient oocytes matured normally and, when fertilized with wild-type sperm, produced healthy appearing offspring, even though miRNA levels were reduced to similar levels as Dicer deficient oocytes. Furthermore, the deletion of both maternal and zygotic Dgcr8 alleles did not impair preimplantation development including the determination of the inner cell mass (ICM) and trophectoderm. Most surprisingly, the mRNA profiles of wild-type and Dgcr8 null oocytes were essentially identical while Dicer null oocytes showed hundreds of misregulated transcripts. These findings show that miRNA function is globally suppressed during oocyte maturation and preimplantation development and that endo-siRNAs, rather than miRNAs, underlie the Dicer knockout phenotype in oocytes.
We used microarrays to understand at the global level how loss of miRNAs and/or siRNAs is impacting mRNA levels in mouse oocytes.
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| Overall design |
9 samples were analyzed, 3 biological replicates each of wild-type, Dgcr8 knockout, and Dicer knockout oocytes
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| Contributor(s) |
Suh N, Blelloch RH |
| Citation(s) |
20054295, 20116247 |
| Submission date |
Jan 14, 2010 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Robert Blelloch |
| E-mail(s) |
blellochr@stemcell.ucsf.edu
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| Phone |
415-476-2838
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| Organization name |
University of California, San Francisco
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| Street address |
513 Parnassus Avenue, Campus Box #0525
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM497293 |
wild-type MII-stage oocytes, biological replicate 1 |
| GSM497294 |
wild-type MII-stage oocytes, biological replicate 2 |
| GSM497295 |
wild-type MII-stage oocytes, biological replicate 3 |
| GSM497296 |
Dgcr8 knockout MII-stage oocytes, biological replicate 1 |
| GSM497297 |
Dgcr8 knockout MII-stage oocytes, biological replicate 2 |
| GSM497298 |
Dgcr8 knockout MII-stage oocytes, biological replicate 3 |
| GSM497299 |
Dicer knockout MII-stage oocytes, biological replicate 1 |
| GSM497300 |
Dicer knockout MII-stage oocytes, biological replicate 2 |
| GSM497301 |
Dicer knockout MII-stage oocytes, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA122039 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19894_RAW.tar |
36.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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