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Series GSE1995 Query DataSets for GSE1995
Status Public on Nov 24, 2004
Title Gene expression in foggy mutant. Guo-1R01NS042626-01A2
Organism Danio rerio
Experiment type Expression profiling by array
Summary The foggy mutant zebrafish show severe deficits in dopaminergic neurons, thus is an important tool to understanding the development of these neurons, which undergo degeneration in Parkinson's disease. Molecular study reveals that the foggy gene encodes a regulator of transcription elongation. Biochemical study suggests that the repressive activity of the Foggy protein is lost in the foggy mutant. The genes whose expression is normally repressed by Foggy are likely to play important roles in dopamine neuron development. However, their identity is unknown. Finding out these target genes of Foggy will provide important insights into dopamine neuron development and regeneration. They will also serve as an entry point to elucidate the role of transcription elongation in controlling neuronal development.
We will identify genes whose expression is altered in the foggy mutant. Their expression pattern and functional significance will be examined in zebrafish through in situ hybridization and morpholino antisense knockdowm methods.
We hypothesize that the genes whose transcription is regulated by Foggy play a critical role in the development of dopaminergic neurons.
The species noted above is Drosophila however we are using ZEBRAFISH. We will examine three stages: 24 hour post fertilization (hpf), 36 hpf, and 48 hpf. During these stages, the dopamine neuron deficits manifest in the foggy mutant. To identify genes whose expression is altered and important for dopamine neuron development, we shall dissect embryonic brain tissues from wildtype and the foggy mutant embryos at the stages mentioned above, and prepare RNA from them. Since the foggy gene is expressed ubiquitously and also required for proper retinal and cardiovascular development, we would like to compare the gene expression between the whole embryos of wildtype and foggy mutant, to identify all possible downstream targets. We are currently applying for a supplement to support the entire microarray experiment. As a pilot experiment, we would like to perform 6 array analysis as soon as possible. Thus, we will examine the 48 hours post fertilzation time point in this pilot experiment and it will be performed in triplicate.
Keywords: other
Contributor(s) Guo S
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Submission date Nov 22, 2004
Last update date Jan 25, 2018
Contact name Winnie Liang
Organization name Translational Genomics
Street address 445 N. Fifth Street
City Phoenix
State/province AZ
ZIP/Postal code 85012
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (4)
GSM35849 brain: 1-WT36h_e1_le1
GSM35850 brain: 1-Foggy36_e1_le1
GSM35851 brain: 2-Foggy36_e1_le1
BioProject PRJNA91013

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Supplementary file Size Download File type/resource
GSE1995_RAW.tar 13.2 Mb (http)(custom) TAR (of CEL)

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