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Series GSE19955 Query DataSets for GSE19955
Status Public on Aug 18, 2010
Title Expression data comparing wild-type and spt mutant zebrafish tissues at two developmental time points.
Organism Danio rerio
Experiment type Expression profiling by array
Summary Mesoderm differentiation in zebrafish relies on a complex interaction between transcription factors and signaling pathways. Tbx16 is a t-box transcription factor involved in this interaction. Here, we examine downstream targets of tbx16 in the intermediate mesoderm at the 4/5-somite stage and tail mesoderm at the 21-somite stage by comparing wild-type tissues with tissues from the tbx16 mutant, spadetail (spt).
 
Overall design We isolated meodermal tissues at two developmental time points (4/5-somite and 21-somite) from both wild-type and spt mutant tissues using dissection and FACS. We compared patterns of gene expression between the mutant and wild-type using Affymetrix zebrafish arrays. We also used GSEA to look for globally disrupted signaling pathways in the mutant at both developmental time points.
 
Contributor(s) Mueller RL, Huang C, Ho RK
Citation(s) 20828405
Submission date Jan 20, 2010
Last update date Jan 25, 2018
Contact name Rachel Lockridge Mueller
E-mail(s) rlm@colostate.edu
Phone (970) 491-6717
Organization name Colorado State University
Department Biology
Street address 1878 Campus Delivery
City Fort Collins
State/province CO
ZIP/Postal code 80523-1878
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (11)
GSM498657 IM1_WT
GSM498658 IM2_WT
GSM498659 IM3_SPT
Relations
BioProject PRJNA122597

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19955_RAW.tar 24.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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