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| Status |
Public on Jan 22, 2010 |
| Title |
Gene expression analysis of Drosophila melanogaster taste tissue |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
To uncover novel molecules involved in taste detection, we performed a microarray-based screen for genes enriched in taste neurons. Proboscis RNA from flies homozygous for a recessive poxn null mutation was compared to RNA from heterozygous controls. Poxn mutants have a transformation of labellar gustatory chemosensory bristles into mechanosensory bristles and therefore lack most or all taste neurons.
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| Overall design |
Proboscises of poxn70 homozygous mutant and poxn70 heterozygous mutant males (8-18 days post eclosure) were dissected, and total RNA was harvested in Trizol according to standard trizol protocol. Samples for each microarray were prepared from 164-280 proboscises. We performed 3 biological replicates for each genotype.
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| Contributor(s) |
Cameron PS, Hiroi M, Ngai J, Scott K |
| Citation(s) |
20364123 |
| Submission date |
Jan 21, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Peter Cameron |
| Organization name |
UC-Berkeley
|
| Street address |
GL71 Koshland, UC-Berkeley
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3200 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| GSM499392 |
poxn homozygous mutant proboscis_rep1 |
| GSM499393 |
poxn homozygous mutant proboscis_rep2 |
| GSM499394 |
poxn homozygous mutant proboscis_rep3 |
| GSM499395 |
poxn heterozygous mutant proboscis_rep1 |
| GSM499396 |
poxn heterozygous mutant proboscis_rep2 |
| GSM499397 |
poxn heterozygous mutant proboscis_rep3 |
|
| Relations |
| BioProject |
PRJNA120143 |
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