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| Status |
Public on Jan 01, 2012 |
| Title |
Expression data of Sorted CD11b+Gr1+ cells from mice with or without LLC tumor challenging |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We sought to identify genes that are differentially regulated in CD11b+Gr1+ cells after tumor challenging. Mice were challenged with LLC-RFP cells (5×10^6 cells, subcutaneous injection) for 9 days. Single cell suspensions were prepared from lungs of both tumor challenged and control wild type mice and stained with antibodies against CD11b and Gr1. Approximately 1×10^5 CD11b+GR1+ myeloid progenitor cells were sorted by FACS (Aria II, BD Bioscience). Total RNA was extracted from the sorted cells for microarray analysis.
In this dataset, we include the expression data of CD11b+Gr1+ cells obtained from both control wild type and tumor challenged mice. These data were used to obtain 22 genes that are upregulated in response to tumor challenging.
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| Overall design |
We analyzed 3 samples from control mice and 2 samples tumor challenged mice. The mean values of the groups were used in the analysis. Genes that were upregulated >2 fold were sorted out and generate the table in the Matrix datasheet.
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| Contributor(s) |
Gao D, Ryu S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 03, 2010 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Dingcheng Gao |
| E-mail(s) |
dig2009@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
CT Surgery
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (5)
|
| GSM506245 |
CD11b+Gr1+ cells from the lung of tumor challenged mice rep1 |
| GSM506246 |
CD11b+Gr1+ cells from the lung of tumor challenged mice rep2 |
| GSM506247 |
CD11b+Gr1+ cells from the lung of control wild type mice rep1 |
| GSM506248 |
CD11b+Gr1+ cells from the lung of control wild type mice rep2 |
| GSM506249 |
CD11b+Gr1+ cells from the lung of control wild type mice rep3 |
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| Relations |
| BioProject |
PRJNA125653 |
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