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| Status |
Public on Dec 21, 2004 |
| Title |
Microarray Based Comparison of three Amplification Methods For Nanogram Amounts of Total RNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Two T7 based methods One round of Amplification (Affymetrix) and Two round of Amplification were compared to two Ribo-SPIA based systems, RiboSPIA and pico Ribo SPIA systems. Data for Pico-RiboSPIA are listed here.
All Hybridisation were performed using Affymetrix Mouse 430-2 gene chips. Data were all scaled to 500.
For 12 chips performed with pico Ribo SPIA the scaling factor average was 2.0 +/- 0.3, background intensities 74.4 +/- 12.7 , noise 3.9 +/- 0.6, rawQ 2.5 +/- 0.3
Keywords: ordered
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| Contributor(s) |
Singh R, Maganti RJ, Jabba SV, Wang M, Deng G, Heath J, Kurn N, Wangemann P |
| Citation(s) |
15613496 |
| Submission date |
Nov 28, 2004 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Philine Wangemann |
| E-mail(s) |
wange@vet.ksu.edu
|
| Phone |
785-532-4863
|
| Organization name |
Kansas State University
|
| Department |
Anatomy and Physiology
|
| Lab |
Cell Physiology Laboratory
|
| Street address |
1600 Dension Avenue
|
| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA91841 |
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