|
| Status |
Public on Oct 08, 2010 |
| Title |
Interleukin-16-knock-out bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We have employed whole genome microarray expression profiling to identify differently expressed genes following stimulation of interleukin-16-knock-out bone-marrow derived macrophages with Escherichia coli lipopolysaccharide (LPS). Macrophages were stimulated with LPS (100 ng/ml) for 6 hours and a signature was identified that distinguished between infected and control samples. Expression of several genes from this signature was quantified in the same RNA samples by real-time PCR, confirming the predicted macrophage response pattern.
|
| |
|
| Overall design |
Gene expression in macrophages was measured at 6 hours after stimulation Three independent experiments were performed.
|
| |
|
| Contributor(s) |
Desnues B, Mège J |
| Citation(s) |
21042409 |
| Submission date |
Feb 05, 2010 |
| Last update date |
May 10, 2018 |
| Contact name |
Benoit Desnues |
| E-mail(s) |
benoit.desnues@univmed.fr
|
| Organization name |
Faculté de Médecine de la Timone
|
| Department |
URMITE UMR CNRS 6236
|
| Street address |
27 boulevard Jean Moulin
|
| City |
Marseille |
| ZIP/Postal code |
13385 cedex 05 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE20210 |
Role of interleukin-16 in macrophage responses to Tropheryma whipplei and lipopolysaccharide |
|
| Relations |
| BioProject |
PRJNA129801 |
Less...
More...