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Series GSE20389 Query DataSets for GSE20389
Status Public on Feb 07, 2011
Title Molecular Characterization of Tannerella forsythia infection-induced bone and soft tissue transcriptional profiles
Organism Mus musculus
Experiment type Expression profiling by array
Summary The objectives of this investigation were to examine changes in the host transcriptional profiles during a Tannerella forsythia infection using a murine calvarial model of inflammation and bone resorption. T. forsythia ATCC 43037 was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and calvarial bones were excised. RNA was isolated from infected soft tissues and calvarial bones and analyzed for transcript profiles using Murine GeneChip® MG-MOE430A Affymetrix arrays to provide a molecular profile of the events that occur following infection of these tissues.
We used mouse microarrays to detail the molecular profile of the events that occur following infection of calvarial and bone tissues and identified distinct classes of up- and down-regulated genes during this process.
 
Overall design T. forsythia was injected at 1.5 x 109 (N = 10) into the soft tissues overlying the calvaria of the mice for 3 days. A control group (N = 9) was injected with vehicle once daily for 3 days. Mice were euthanized 8 h after the last injection. The calvarial bones and overlying soft tissues from 5 mice in each group were excised, snap frozen in liquid nitrogen, and stored at –80°C until RNA isolation. Total RNA was isolated from the frozen calvarial tissue and calvarial bone from each mouse (T. forsythia infected and control animals, N = 3-5 in each group) with Trizol reagent (Invitrogen, CA). Equal amounts of RNA from samples were labeled and hybridized on a mouse GeneChip following the protocol described in the GeneChip Expression Analysis Technical manual (Affymetrix, Santa Clara, CA). After hybridization, the GeneChip arrays were stained and scanned in an Affymetrix GCS 3000 7G Scanner.
 
Contributor(s) Kesavalu LN, Bakthavatchalu V, Meka A, Sathishkumar S, Lopez CM, Verma RK, Wallet SM, Bhattacharyya I, Boyce BF, Mans JJ, Lamont RJ, Baker HV, Ebersole JL
Citation(s) 20883221
Submission date Feb 17, 2010
Last update date Jan 08, 2019
Contact name Kesavalu Naidu Lakshmyya
Organization name University of Florida, College of Dentistry
Department Periodontology
Lab Oral Microbiology
Street address 1600 SW Archer road, D11-24
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (17)
GSM463608 Control soft tissue 1
GSM463609 Control soft tissue 2
GSM463610 Control soft tissue 3
Relations
BioProject PRJNA125513

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20389_RAW.tar 55.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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