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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 10, 2010 |
| Title |
Mammary epithelial cell subpopulations from control and ovariectomized mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To study the effect of pregnancy on mouse mammary epithelial subpopulations, epthelial cells derived from control or ovariectomized adult mice were isolated using fluorescence-activated cell sorting. After elimination of haematopoietic and endothelial cells, two distinct epithelial subpopulations were sorted using antibodies against CD29 and CD24. Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as mammary stem cell enriched (CD29hiCD24+) and luminal (CD29loCD24+) respectively (ref: Shackleton et al, Nature 2006). Microarray profiling was used to compare gene expression profiles of the two subpopulations isolated from ovariectomized and control mice.
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| Overall design |
For each of two biological replicates, 6 FVB/NJ mice were ovariectomized at 8 weeks of age. 4 weeks after ovariectomy (Ovx) mammary gland from control or Ovx animals were collected and digested to obtain a single cell suspension. CD45-CD31-TER119- cells were then sorted based on the expression of cell surface markers CD24 and CD29. Similarly for two pools of control mice. There were also 4 technical replicates, to make 12 BeadChips in total.
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| Contributor(s) |
Smyth GK, Wu D, Asselin-Labat M, Lindeman GJ, Visvader JE |
| Citation(s) |
20383121 |
| Submission date |
Feb 18, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
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| Phone |
(+61 3) 9345 2326
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| Fax |
(+61 3) 9347 0852
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| URL |
http://www.wehi.edu.au
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| Organization name |
Walter and Eliza Hall Institute of Medical Research
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| Department |
Bioinformatics
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| Lab |
Smyth
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| Street address |
1G Royal Pde
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| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| GSM511113 |
Ovx, MaSC-enriched, replicate 1 |
| GSM511114 |
Control, MaSC-enriched, replicate 2 |
| GSM511115 |
Ovx, MaSC-enriched, replicate 2 |
| GSM511116 |
Ovx, Luminal, replicate 2 |
| GSM511117 |
Ovx, MaSC-enriched, technical replicate of replicate 2 |
| GSM511118 |
Control, Luminal, replicate 2 |
| GSM511119 |
Control, MaSC-enriched, technical replicate of replicate 2 |
| GSM511120 |
Control, Luminal, technical replicate of replicate 1 |
| GSM511121 |
Ovx, Luminal, techinical replicate of replicate 1 |
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| This SubSeries is part of SuperSeries: |
| GSE22446 |
Control of mammary stem cell function by steroid hormone signalling. |
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| Relations |
| BioProject |
PRJNA129827 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20401_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE20401_non_normalized.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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