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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 10, 2010 |
| Title |
Mouse mammary epithelial cell subpopulations from pregnant and virgin mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To study the effect of pregnancy on mouse mammary epithelial subpopulations, epthelial cells derived from virgin or pregnant (12.5 day pregnant) mice were isolated using fluorescence-activated cell sorting. After elimination of haematopoietic and endothelial cells, two distinct epithelial subpopulations were sorted using antibodies against CD29 and CD24. Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as mammary stem cell enriched (CD29hiCD24+) and luminal (CD29loCD24+) respectively (ref: Shackleton et al, Nature 2006). Microarray profiling was used to compare gene expression profiles of the two subpopulations in 12.5 day pregnant and virgin mice.
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| Overall design |
For each biological replicate, mammary gland from virgin or 12.5 day pregnant FVB/NJ mice were collected and digested to obtain a single cell suspension. CD45-CD31-TER119- cells were then sorted based on the expression of cell surface markers CD24 and CD29. There were 4 pools of pregant mice and 3 pools of virgin mice.
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| Contributor(s) |
Smyth GK, Wu D, Asselin-Labat M, Lindeman GJ, Visvader JE |
| Citation(s) |
20383121 |
| Submission date |
Feb 18, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
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| Phone |
(+61 3) 9345 2326
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| Fax |
(+61 3) 9347 0852
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| URL |
http://www.wehi.edu.au
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| Organization name |
Walter and Eliza Hall Institute of Medical Research
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| Department |
Bioinformatics
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| Lab |
Smyth
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| Street address |
1G Royal Pde
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| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (14)
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| GSM511125 |
12.5 dP, luminal, replicate 2 |
| GSM511126 |
Virgin, MaSC-enriched, replicate 2 |
| GSM511127 |
Virgin, luminal, replicate 2 |
| GSM511128 |
12.5 dP, luminal, replicate 3 |
| GSM511129 |
12.5 dP, MaSC-enriched, replicate 1 |
| GSM511130 |
12.5 dP, MaSC-enriched, replicate 2 |
| GSM511131 |
12.5 dP, MaSC-enriched, replicate 3 |
| GSM511132 |
Virgin, MaSC-enriched, replicate 3 |
| GSM511133 |
Virgin, luminal, replicate 3 |
| GSM511134 |
12.5 dP, luminal, replicate 4 |
| GSM511135 |
12.5 dP, MaSC-enriched, replicate 4 |
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| This SubSeries is part of SuperSeries: |
| GSE22446 |
Control of mammary stem cell function by steroid hormone signalling. |
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| Relations |
| BioProject |
PRJNA129829 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20402_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE20402_non_normalized.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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