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| Status |
Public on May 31, 2022 |
| Title |
Temporally-Divergent Regulatory Mechanisms Govern Neuronal Diversification and Maturation in the Neocortex [scATAC-seq] |
| Organisms |
Callithrix jacchus; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Mammalian neocortical neurons span one of the most diverse cell type spectra of any tissue. The regulatory strategies that neurons use during progressive development and maturation remain unclear. We present an integrated single-cell epigenomic and transcriptional analysis of individual classes of neurons from both mouse and marmoset neocortex, sampled during both early postmitotic stages of identity acquisition and later stages of neuronal plasticity and circuit integration. We find that in both species, the regulatory strategies controlling early and late stages of pan-neuronal development diverge. Early postmitotic neurons use molecular regulatory programs with broader tissue distribution and greater evolutionary conservation. In contrast, programs that are active during later neuronal maturation implement more brain- and neuron-specific mechanisms showing greater evolutionary divergence. Our work uncovers a temporally-regulated shift in regulatory choices, likely reflecting unique evolutionary constraints on distinct events of neuronal development in the neocortex.
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| Overall design |
Examination of the regulatory strategies governing neuronal development in mouse and marmoset neocortex across timepoints
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| Contributor(s) |
Yuan W, Ma S, Brown JR, Kim K, Murek V, Trastulla L, Meissner A, Lodato S, Shetty A, Levin JZ, Buenrostro JD, Ziller MJ, Arlotta P |
| Citation(s) |
35915179 |
| Submission date |
May 24, 2022 |
| Last update date |
Aug 30, 2022 |
| Contact name |
Paola Arlotta |
| E-mail(s) |
paola_arlotta@harvard.edu
|
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
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| Lab |
Arlotta
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL21580 |
Illumina HiSeq 2500 (Callithrix jacchus) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE204851 |
Temporally-Divergent Regulatory Mechanisms Govern Neuronal Diversification and Maturation in the Neocortex |
|
| Relations |
| BioProject |
PRJNA842149 |
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