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Series GSE20518 Query DataSets for GSE20518
Status Public on Feb 24, 2010
Title mRNA expression in islets isolated from wild type and SHPKO mice fed chow or western diet
Organism Mus musculus
Experiment type Expression profiling by array
Summary SHP (small heterodimer partner; NR0B2) belongs to the nuclear hormone receptor superfamily, which regulates numerous developmental and metabolic cellular functions. To study physiological function of SHP, we generated congenic SHP-/- mice on C57Bl/6 background. When the congenic SHP-/- mice were challenged with a western diet (high fat, hgih sucrose, high cholesterol) for 20 weeks, they were resistant to diet induced obesity but severely glucose intolerant compared to wild type control mice. However, their overall peripheral tissue insulin sensitivity was normal when assessed by insulin tolerance test. Next, we examined the glucose stimulated insulin secretion (GSIS) in isolated islets from these animals. Islets from SHP-/- mice showed strongly impaired GSIS especially fed the western diet, which is believed to be a major factor causing the whole body glucose intolerance in SHP-/- mice. Therefore, we explored gene expression in islets using illumina beadchip array to understand mechanisms underneath the impaired GSIS.
 
Overall design Islets were isolated from C57Bl/6 wild type and C57Bl/6 SHP-/- mice fed chow or western diet. The same amount of total RNA obtained from these islets was pooled from 5-9 mice each group and subjected to illumina beadchip gene expression profiling in triplicates.
 
Contributor(s) Lee YK, Moore DD
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Submission date Feb 24, 2010
Last update date Jan 16, 2019
Contact name Yoonkwang Lee
E-mail(s) ylee3@neomed.edu
Organization name Northeast Ohio Medical University
Department Integrative Medical Sciences
Street address 4209 State Route 44
City Rootstown
ZIP/Postal code 44272
Country USA
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (12)
GSM515390 WT_Chow_rep1
GSM515391 WT_Chow_rep2
GSM515392 WT_Chow_rep3
Relations
BioProject PRJNA125243

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20518_RAW.tar 15.8 Mb (http)(custom) TAR
GSE20518_matrix_non-normalized.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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