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| Status |
Public on Jun 20, 2022 |
| Title |
Effects of triiodothyronine on human osteoblast-like cells: novel insights from a global transcriptome analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to investigate, at the mRNA level, the signaling pathways through which triiodothyronine (T3) affects osteoblast function, human mesenchymal stem cells derived from adipose tissue were subjected to a pre-established osteoinduction protocol, resulting in osteoblast-like cells, which were cultured with or without T3. RNA-Seq was performed using Illumina platform, and differential gene expression was assessed with DESeq2. Among differentially expressed genes, enrichment analysis was performed for biological processes against the Gene Ontology Consortium database, using both ClusterProfiler R package and STRING.
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| Overall design |
The study consists of three groups with three biological replicates each. Osteoblast-like cells were cultured for 72h with no T3 for the control group, and 1nM or 10nM T3 for the remaining groups.
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| Contributor(s) |
Rodrigues BM, Mathias LS, Deprá IC, Cury SS, Oliveira M, Olímpio RM, Síbio MT, Gonçalves BM, Nogueira CR |
| Citation(s) |
35784485 |
| Submission date |
Jun 08, 2022 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Célia Regina Nogueira |
| Organization name |
Faculdade de Medicina de Botucatu - Unesp
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| Street address |
Av. Prof. Mário Rubens Guimarães Montenegro, s.n.
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| City |
Botucatu |
| ZIP/Postal code |
18618-687 |
| Country |
Brazil |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA847172 |
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