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| Status |
Public on Jun 20, 2022 |
| Title |
Transcriptome sequencing of triple-matched samples of gastric cancer patients with ovarian metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our aim was to decipher the underlying molecular mechanism of synchronous ovarian metastasis of gastric cancer. We hereby conducted transcriptome sequencing of triple-matched samples including normal gastric mucosa, primary gastric cancer and ovarian metastatic tumors from 3 individual patients with the application of Illumina sequencing platform with 150-bp paired-end. Follow-up analyses not only identified differentially expressed genes between different sample sets (a threshold of fold change >2 and adjusted P value <0.05) but also uncovered significantly enriched signaling pathways of individual type. To sum up, our comparative transcriptomic analyses of triple-matched fresh samples stored in liquid nitrogen profiled the molecular expression and revealed functionally enriched pathways underlying the ovarian metastasis of gastric cancer.
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| Overall design |
Transcriptomic profiling of triple-matched primary gastric cancer, normal mucosae and ovarian metastasis with the application of deep sequencing via Illumina Hiseq.
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| Contributor(s) |
Gao J, Xu D, Huang S |
| Citation missing |
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| Submission date |
Jun 17, 2022 |
| Last update date |
Jun 20, 2022 |
| Contact name |
Shenglin Huang |
| Organization name |
Fudan University
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| Street address |
270 Dongan
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA850175 |
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