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| Status |
Public on Jun 24, 2022 |
| Title |
Expression profiles of lncRNAs in 3D cultures. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Breast cancer cells reprogram the oncogenic lncRNAs/mRNAs co-expression networks in three- dimensional microenvironment
To have a more functional approach, organotypic 3D cell cultures that more accurately mimic the characteristics of solid tumors in vivo and the tumor microenvironment are required. In this study, DNA microarrays were employed to deline the changes in lncRNAs expression patterns of breast cancer cells, cultured in 3D and 2D conditions from BT-474 cell line. Furthermore, potential lncRNAs/mRNAs pairs co-expressed in 3D cultures exhibit a high degree of similarity with those found in luminal B breast cancer patients suggesting that they could be adequate pre-clinical tools to identify, not only biomarkers related to endocrine therapy response and PCR, but to understand the biological behavior of cancer cells in 3D microenvironments, which point towards an important contribution of the roles of lncRNAs in organotypic 3D cultures.
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| Overall design |
We analyzed 3D culture samples compared to 2D cultures of the BT-474 luminal B cell line, using the human Clariom D array plataform. Array data were processed with the Affymetrix GeneChip command console software v4.0 computational tool. Samples were run in triplicate.
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| Contributor(s) |
Nuñez-Olvera SI, Aguilar-Arnal L, Marchat AL, Cisneros-Villanueva M, Hidalgo-Miranda A, Mauricio-Dorantes A, Salinas-Vera MY, Ramos-Payán R, Pérez-Plasencia C, Carlos-Reyes A, Puente-Rivera J, López-Camarillo C |
| Citation(s) |
36359853 |
| Submission date |
Jun 23, 2022 |
| Last update date |
Nov 30, 2022 |
| Contact name |
Stephanie Iraiz Nuñez Olvera |
| E-mail(s) |
blackvolkova@gmail.com
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| Phone |
5569161152
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| Organization name |
UACM
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| Department |
Ciencias Genomicas
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| Lab |
oncogenomica del cancer
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| Street address |
S. Lorenzo 290, Col del Valle Sur, Benito Juárez, 03100 Ciudad de México, CDMX
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| City |
CDMX |
| ZIP/Postal code |
03100 |
| Country |
Mexico |
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| Platforms (1) |
| GPL24539 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [probe set (exon) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA852280 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206836_RAW.tar |
317.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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