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Series GSE20707 Query DataSets for GSE20707
Status Public on Jun 24, 2010
Title Detection of the up-regulated and down-regulated genes in stac mutant of zebrafish by cDNA microarray
Organism Danio rerio
Experiment type Expression profiling by array
Summary We performed a zebrafish forward genetic screen by Tol2 mediated gene-trap approach and uncovered one mutant stac (The number of the transgenic line: B55) that showed severe cell-death distributed in the various brain and trunk in the homozygote embryos. Analysis of stac homozygous embryos demonstrates typical apoptosis. So it is necessary to analyze whether the apoptosis and cell cycle regulated signaling transductions are changed in the mutant, in order to provide valuable clues to some other species. And the up-regulated and down-regulated genes in the mutant compared to the WT were examined by zebrafish cDNA microarray.
 
Overall design Total RNA was isolated from wild-type and mutant embryos in 24hpf and 30hpf and their quality was checked by the company with LAB-ON-A –CHIP system. The reverse transcription and Biotin-labeling has been done with the RNA as template. The hybridization and elution was performed with Affymetrix GeneChip® Zebrafish Genome Array. Both the control group (wild-type) and the experimental group (mutant) have two repeats.
 
Contributor(s) Zhao X, Zhao L, Tian T, Zhang Y, Tong J, Zheng X, Meng A
Citation(s) 20573960
Submission date Mar 09, 2010
Last update date Jan 25, 2018
Contact name Xinyi Zhao
E-mail(s) zhaoxy04@mails.tsinghua.edu.cn
Phone 861062771164
Fax 861062794401
Organization name Tsinghua University
Department Department of Biological Sciences and Biotechnology
Lab Molecular and developmental biology laboratory
Street address Haidian District, Tsinghuayuan, No.1
City Beijing
ZIP/Postal code 100084
Country China
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (8)
GSM519692 wild type embryos 24hpf, biological rep1
GSM519693 wild type embryos 24hpf, biological rep2
GSM519694 stac embryos 24hpf, biological rep1
Relations
BioProject PRJNA124903

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20707_RAW.tar 17.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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