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| Status |
Public on Mar 10, 2011 |
| Title |
Gene expression analysis of miR-663 knockdown under shear stress in HUVEC |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The mechanisms by which oscillatory shear (OS) induces, while high laminar shear stress (LS) prevents, atherosclerosis are still unclear. Here, we examined the hypothesis that OS induces inflammatory response, a critical atherogenic event, in endothelial cells by a miRNA-dependent mechanism. In particular, miR-663 mediated OS-induced inflammation through monocyte adhesion to endothelial cells. The potential targets of miR-663 were examined by knockdown of miR-663 and then the gene expression profiles were determined under shear stress conditions.
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| Overall design |
To determine the potential targets of miR-663, HUVEC were transfected either with miR-663-LNA (inhibitor of miR-663) or control-LNA and then subjected to OS or LS for 24hr. Total RNA were collected from these four groups and microarray studies (Illumina HumanHT-12 v3 beadchip) were performed.
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| Contributor(s) |
Jo H, Ni C |
| Citation(s) |
21378144 |
| Submission date |
Mar 10, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Hanjoong Jo |
| E-mail(s) |
hanjoong.jo@bme.gatech.edu
|
| Phone |
404-712-9654
|
| Fax |
404-727-3330
|
| Organization name |
Emory University
|
| Department |
Biomedical Engineering
|
| Street address |
2005 WMB
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30068 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA124979 |
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