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Series GSE20878 Query DataSets for GSE20878
Status Public on Nov 10, 2010
Title Hepatic gene expression profiling reveals key pathways involved in leptin mediated weight loss in ob/ob mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The purpose of this study was to identify leptin target genes and subsequent pathways correlated with leptin-mediated weight loss. We utilized the microarray technology to compare two types of leptin administration: one involving a direct stimulatory effect when administered peripherally (subcutaneous: SQ) and another that is indirect, involving a hypothalamic relay that suppresses food intake when leptin is administered centrally (intracerebroventricular: ICV). We report here the impact of central and peripheral administration of leptin on food intake, body weight and body fat composition in ob/ob mice. We also report hepatic gene expression changes caused by central versus peripheral leptin administration.

Keywords: comparison
Overall design Leptin deficient (ob/ob) mice were continuously administered leptin over 12-days using central (intracerebroventricular) or peripheral (subcutaneous) route of administration. Liver RNA was extracted and hybridized to Illumina microarrays and gene expression data was analyzed. The global gene expression profiles were compared after the central and peripheral leptin treatments in ob/ob mice and C57BL6 mice were used for the baseline gene expression. The groups are as below:
Liver_B6: C57BL6 mice
Liver_VEH_SQ: ob/ob mice with vehicle subcutaneous treatment
Liver_LEP_SQ: ob/ob mice with leptin subcutaneous treatment
Liver_VEH_ICV: ob/ob mice with vehicle intracerebroventricular treatment,
Liver_LEP_ICV: ob/ob mice with leptin intracerebroventricular treatment,
Liver_LEP_ICVN: represents four animals from LEP_ICV group in which treatment failed or the cannula may not have been in place. While analyzing the phenotype data, we found that there was no weight loss in these four animals. Sectioning of the brain could not confirm placement of the cannula in these animals. The expression of these animals is very similar to the vehicle treated animals.
Contributor(s) Sharma A, Bartell S, Baile C, Chen B, Podolsky R, McIndoe R, She J
Citation(s) 20808936
Submission date Mar 15, 2010
Last update date Jun 14, 2018
Contact name Jin-Xiong She
Phone 706-721-3410
Fax 706-721-3688
Organization name Medical College of Georgia
Department Center for Biotechnology and Genomic Medicine
Street address 1120 15th Street, CA4124
City Augusta
State/province GA
ZIP/Postal code 30912-2400
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (26)
GSM522049 Liver_B6_5541
GSM522050 Liver_B6_5542
GSM522051 Liver_B6_5543
BioProject PRJNA124675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20878_RAW.tar 3.1 Mb (http)(custom) TAR
GSE20878_non-normalized.txt.gz 2.7 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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