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| Status |
Public on Jul 22, 2022 |
| Title |
IL-9 exacerbates SARS-CoV2 infection and associated airway inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus diseases 2019 (COVID-19) and broncho-alveolar inflammation (Merad and Martin, 2020). IL-9 induces airway inflammation and bronchial hyper responsiveness in respiratory viral illnesses and allergic inflammation (Temann et al., 1998). However, the role of IL-9 is not yet identified in SARS-CoV2 infection. Here we show that IL-9 promotes SARS-CoV2 infection and airway inflammation in K18-hACE2 transgenic (ACE2.Tg) mice, as IL-9 blockade reduces SARS-CoV2 infection and suppressed airway inflammation. Foxo1 is essential for the induction of IL-9 in helper T (Th) cells (Malik et al., 2017). While ACE2.Tg mice with Foxo1-deficiency in CD4+ T cells were performed to be resistant to SARS-CoV2 infection associated with reduced IL-9 production, exogenous IL-9 made Foxo1-deficient mice susceptible to SARS-CoV2 infection with increased airway inflammation. Collectively, we identify a mechanistic insight of IL-9-mediated regulation of antiviral and inflammatory pathways in SARS-CoV2 infection, and unravel a principle for the development of host-directed therapeutics to mitigate disease severity.
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| Overall design |
We isolated the RNA from lung tissues of infected Foxofl/flxCD4Cre+ and CD4Cre- mice and handedover to the bionivid pvt ltd. For further sequencing.
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| Contributor(s) |
Sadhu S, Dalal R, Dandotiya J, Binayke A, Singh V, Tripathy MR, Das V, RIZVI ZA |
| Citation missing |
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| BioProject |
PRJNA842504 |
| Submission date |
Jul 22, 2022 |
| Last update date |
Jul 22, 2022 |
| Contact name |
Srikanth Sadhu |
| E-mail(s) |
srikanthsadhu@thsti.res.in
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| Organization name |
Transational health science and Technology institute
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| Street address |
Faridabad Gurugram Expressway
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| City |
Faridabad |
| State/province |
Haryana |
| ZIP/Postal code |
121001 |
| Country |
India |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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