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Series GSE20984 Query DataSets for GSE20984
Status Public on Mar 21, 2010
Title Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone
Organism Mus musculus
Experiment type Expression profiling by array
Summary We investigated the Prkar1a+/- mice when bred with Prkaca+/- genetic backgrounds. Prkar1a+/-Prkaca+/- mice not only continued to develop bone lesions but also demonstrated a significant increase in both the number and the severity of the lesions, as well as a reduction in the age of first appearance of any bone abnormality. Histological analysis of bone from Prkar1a+/-Prkaca+/- mice showed that lesions had similarity to tumors from humans with CNC and some resemblance (but also differences) from humans and mice with fibrous dysplasia (FD). Prkar1a+/- and Prkar1a+/-Prkaca+/- mouse lesions always involved a particular sub-population of cells that could be identified as belonging to the osteoblastic lineage, that were initially located on the endosteal surface of the periosteum and nearby trabecular bone proximal to the growth plate of the long bones and vertebrae, and resembled bone stromal cells (BSCs). The bone lesions expressed high level of mesenchymal proteins like n-cadherin, vimentin, snail1, twist1, mmp2 and mmp9 when compared to WT bone. On top of this, bone lesions from Prkar1a+/-Prkaca+/- mice also showed an increased expression of keratin related genes, which are involved in hair follicle and epithelial differentiation, when compared to lesions from Prkar1a+/- mice.
Overall design Total RNA obtained from bone lesions from Prkar1a+/- and Prkar1a+/-Prkaca+/- mice were compared to those obtained from WT and Prkaca+/- mice.
Contributor(s) Tsang KM, Nesterova M, Stratakis CA, Cheadle C, Watkins T
Citation(s) 20421483
Submission date Mar 20, 2010
Last update date Jun 14, 2018
Contact name Kitman Tsang
Phone 301-451-8250
Fax 301-402-0574
Organization name SEGEN/PDEGEN/NICHD/NIH
Street address 10 Center Drive, 10-CRC, Rm1E-3216
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (13)
GSM524645 WT caudal vertebral bone, rep1
GSM524646 WT caudal vertebral bone, rep2
GSM524647 WT caudal vertebral bone, rep3
BioProject PRJNA124493

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20984_RAW.tar 3.1 Mb (http)(custom) TAR
GSE20984_non-normalized.txt.gz 1.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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