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Series GSE21083 Query DataSets for GSE21083
Status Public on Oct 29, 2010
Title Benefits of a 6 week supplementation of sebacic acid on a mouse model of type 2 diabetes (db/db mice)
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study aimed at investigating the impact of chronic ingestion of sebacic acid (SA), a 10 carbons medium-chain dicarboxylic acid, on glycemic control in a mouse model of type 2 diabetes (db/db mice). Three groups of 15 mice were fed for 6 weeks either a chow diet (Ctrl), or a chow diet supplemented with 1.5% or 15% (SA1.5% and SA15% resp.) energy from SA. Fasting glycemia was measured once a week and HbA1c before and after supplementation. An oral glucose tolerance test (OGTT) was performed at the end of the supplementation. Gene expression was determined by transcriptomic analysis on the liver of the Ctrl and SA15% groups. Results-After 42 days of supplementation, fasting glycemia and HbA1c were ~70% and ~25% lower in the SA15% group compared to other groups showing a beneficial effect of SA on hyperglycemia. During OGTT, blood glucose area under the curve (AUC) was reduced after SA15% compared to other groups. This effect was associated with a tendency for an improved insulin response. In the liver, Pck1 and FBP mRNA were statistically decreased in the SA15% compared to Ctrl suggesting a reduced hepatic glucose output induced by SA. Conclusions-Dietary supplementation of SA largely improves glycemic control in a mouse model of type 2 diabetes. This beneficial effect may be due (1) to a reduced hepatic glucose output resulting from transcriptional down regulation of key gluconeogenesis genes and (2) to an improved glucose induced-insulin secretion.
 
Overall design Microarray analysis of 6-8 wk old male BKS.Cg-m+/+Leprdb/J 000642 db/db mice. 2 groups. n=15/group:
1) Control group.
2) Sebacic acid high dose group - 15% (77.6g/kg food, ≈9g/kg body weight per day).
 
Contributor(s) Binnert C, Raymond F, Membrez M, Metairon S
Citation(s) 20977585
Submission date Mar 26, 2010
Last update date Jun 14, 2018
Contact name Frederic Raymond
E-mail(s) frederic.raymond@rd.nestle.com
Organization name Nestle Institute of Health Sciences
Department Functional Genomics
Street address Campus EPFL - Quartier de l'Innovation
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (30)
GSM527058 Control Liver 01
GSM527059 Control Liver 02
GSM527060 Control Liver 03
Relations
BioProject PRJNA126723

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21083_RAW.tar 3.1 Mb (http)(custom) TAR
GSE21083_non-normalized_data.txt.gz 4.8 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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