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| Status |
Public on Apr 05, 2010 |
| Title |
OCT4 Knockdown in human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We studied the genomic locations of three key regulatory proteins (OCT4, NANOG and CTCF) in human and mouse embryonic stem (ES) cells [see Series GSE20650]. To identify the conserved and unique human OCT4 targets, we performed an OCT4 RNAi knock-down experiment. We find that species-specific transposable elements have profoundly altered the transcriptional circuitry of pluripotent stem cells.
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| |
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| Overall design |
To identify the mRNA targets of OCT4:
12 samples were collected in total. Each sample is done in triplicate and samples were collected at 2 different time point, 2 days and 3 days after transfection. Luciferase knockdown serves as negative control and OCT4 knockdown is the experimental sample.
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| |
|
| Contributor(s) |
Bourque G, Ng H |
| Citation(s) |
20526341 |
| Submission date |
Mar 30, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Justin Jeyakani Joseph Gnanakkan |
| E-mail(s) |
gnanakkan@gis.a-star.edu.sg
|
| Phone |
68088173
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Computational and Systems Biology
|
| Street address |
60, Biopolis street
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (12)
|
| GSM528603 |
Luciferase shRNA_3 Days knockdown_rep1 |
| GSM528604 |
OCT4 shRNA_3 Days knockdown_rep1 |
| GSM528605 |
Luciferase shRNA_2 Days knockdown_rep2 |
| GSM528606 |
OCT4 shRNA_2 Days knockdown_rep2 |
| GSM528607 |
Luciferase shRNA_2 Days knockdown_rep1 |
| GSM528608 |
OCT4 shRNA_2 Days knockdown_rep1 |
| GSM528609 |
Luciferase shRNA_3 Days knockdown_rep3 |
| GSM528610 |
OCT4 shRNA_3 Days knockdown_rep3 |
| GSM528611 |
Luciferase shRNA_2 Days knockdown_rep3 |
| GSM528612 |
OCT4 shRNA_2 Days knockdown_rep3 |
| GSM528613 |
Luciferase shRNA_3 Days knockdown_rep2 |
| GSM528614 |
OCT4 shRNA_3 Days knockdown_rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE21200 |
OCT4, NANOG and CTCF in human embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA129669 |
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