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Series GSE211412 Query DataSets for GSE211412
Status Public on Aug 23, 2022
Title Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis [Acipenser ruthenus]
Organism Acipenser ruthenus
Experiment type Expression profiling by high throughput sequencing
Summary In this study we analyzed the spatial and temporal localization of maternal transcripts during oogenesis in Acipenser ruthenus. The occurrence of transcript asymmetry in A. ruthenus has been described at a global level only in matured eggs. However not much is known about the asymmetry during oogenesis. In this study we assessed the temporal establishment of the transcript localization at a global level for A. ruthenus. We were able to determine that there are many transcripts that show temporal variability in the establishment of their localization. We observed an early, predefined and also late pathways for both the vegetally and animally localized transcripts. Additionally, we showed that some maternal transcripts are dynamic during oogenesis with degradation and de novo production being observed. Our study showed that in additional to spatial orientation to the transcripts, there is a strong temporal factor. The discovery of these new temporal profiles should help to better understand the driving forces during embryogenesis.
 
Overall design Oocytes from Acipenser ruthenus females were extracted to create two groups: small oocytes (n = 5; size 1320-1500 µm); and big (n = 5, size 1920-2100 µm). Samples were oriented along their animal-vegetal axis and cryosectioned to produce 5 main sections (A (extreme animal) – E (extreme vegetal)). Each section was sequenced and the abundance of the transcript across the section and stages analyzed.
 
Contributor(s) Abaffy P, Iegorova V, Naraine R, Sindelka R
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Submission date Aug 16, 2022
Last update date Aug 23, 2022
Contact name Radek Sindelka
E-mail(s) Radek.Sindelka@ibt.cas.cz
Organization name Institute of Biotechnology, Czech Academy of Science, v.v.i.
Lab Laboratory of Gene Expression
Street address Průmyslová 595
City Vestec by Prague
ZIP/Postal code 25250
Country Czech Republic
 
Platforms (1)
GPL26119 Illumina NextSeq 500 (Acipenser ruthenus)
Samples (35)
GSM6469664 section A big oocyte rep 1 [A. ruthenus]
GSM6469665 section B big oocyte rep 1 [A. ruthenus]
GSM6469666 section C big oocyte rep 1 [A. ruthenus]
This SubSeries is part of SuperSeries:
GSE211416 Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis
Relations
BioProject PRJNA870172

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE211412_aruthenus_alteration_between_the_sections_at_big_stage_normalized.xlsx 7.7 Mb (ftp)(http) XLSX
GSE211412_aruthenus_alteration_between_the_sections_at_small_stage_normalized.xlsx 7.8 Mb (ftp)(http) XLSX
GSE211412_aruthenus_altered_magnitudes_per_section_and_stage_normalized.xlsx 16.8 Mb (ftp)(http) XLSX
GSE211412_aruthenus_non_normalized.xlsx 6.9 Mb (ftp)(http) XLSX
GSE211412_aruthenus_total_altered_counts_per_stage_non_normalized.xlsx 2.1 Mb (ftp)(http) XLSX
GSE211412_aruthenus_total_altered_counts_per_stage_normalized.xlsx 4.3 Mb (ftp)(http) XLSX
GSE211412_sterlet_transcriptome_merged.fasta.gz 13.4 Mb (ftp)(http) FASTA
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