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Series GSE21162 Query DataSets for GSE21162
Status Public on Jun 16, 2010
Title Expression data from the PVD and OLL neurons in C. elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Nociceptive neurons develop a complex dendritic arbor to sense noxious stimuli, which enables animals to react to environmental insults and perform self-protective behaviours. The genetic programs controlling neuronal dendritic morphogenesis are poorly understood. In C. elegans, the PVD sensory neuron generates a complex dendritic arbor that envelops the body of the animal. This nociceptive neuron enables study of dendrite formation in vivo.
We used expression arrays to identify genes highly enriched in the PVD neuron while undergoing dendritic morphogenesis. These genes could function in pathways controlling dendrite formation.
 
Overall design We employ the mRNA-tagging method to isolate poly(A) RNA from the PVD and OLL neurons by expressing a 3X FLAG-tagged poly(A) binding protein PAB-1 in PVD and OLL under control of the ser-2prom3B promoter. The enriched poly(A) RNA is amplified using the NuGEN WT-Pico amplification system and applied to Affymetrix C. elegans gene expression microarrays. A reference sample was obtained by isolating total RNA from whole animals at the L3/L4 stages and processed as above.
 
Contributor(s) Watson JD, Smith CJ, Spencer WC, O'Brien T, Cha B, Albeg A, Treinin M, Miller III DM
Citation(s) 20537990
Submission date Apr 01, 2010
Last update date Jul 06, 2016
Contact name William Clay Spencer
E-mail(s) clip78@gmail.com
Phone 4409356643
Organization name Vanderbilt University
Department Cell & Developmental Biology
Lab David Miller
Street address 465 21st Ave S
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (6)
GSM529725 PVD and OLL neurons, biological rep1
GSM529726 PVD and OLL neurons, biological rep2
GSM529727 PVD and OLL neurons, biological rep3
Relations
BioProject PRJNA126615

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Supplementary file Size Download File type/resource
GSE21162_RAW.tar 10.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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